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Rabbit Anti-NKAP  antibody (bs-12400R)  
~~~促銷代碼KT202411~~~
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說明書: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價

產(chǎn)品編號 bs-12400R
英文名稱 Rabbit Anti-NKAP  antibody
中文名稱 NFKB激活蛋白抗體
別    名 NF-kappa-B-activating protein; Nkap; NKAP_HUMAN; Nuclear NF kappaB activating protein; Nuclear NF kappa B activating protein; NFkB activating protein.  
研究領域 細胞生物  染色質(zhì)和核信號  信號轉(zhuǎn)導  干細胞  轉(zhuǎn)錄調(diào)節(jié)因子  淋巴細胞  t-淋巴細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse (predicted: Human,Rat,Rabbit,Sheep,Dog,Horse)
產(chǎn)品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 47kDa
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human NKAP: 321-415/415 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 NFκB, a pleiotropic transcription factor, is present in almost all cell types and is involved in many biological processes including inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFκB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFκB1/p105, NFκB1/p50, REL and NFκB2/p52. This complex is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NKAP (NF-κ-B-activating protein) is a 415 amino acid nuclear protein that regulates TNF and IL1-induced NFκB activation. As component of a DNA-binding complex, NKAP also functions as a transcriptional repressor that acts on NOTCH target genes. Loss of NKAP blocks the development of αβT- cells, suggesting that it is required for their maturation through repression of NOTCH genes.

Function:
Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter.

Subunit:
Component of the Notch corepressor complex. Interacts with CIR1 and HDAC3.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity:
Belongs to the UPF0396 family.

SWISS:
Q8N5F7

Gene ID:
79576

Database links:

Entrez Gene: 79576 Human

Omim: 300766 Human

SwissProt: Q8N5F7 Human

Unigene: 522771 Human



產(chǎn)品圖片
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NKAP Polyclonal Antibody, Unconjugated(bs-12400R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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