產(chǎn)品編號(hào) | bs-12441R |
英文名稱 | Rabbit Anti-ABCF2 antibody |
中文名稱 | ATP結(jié)合盒轉(zhuǎn)運(yùn)蛋白2抗體 |
別 名 | 0710005O05Rik; ABC28; ABCF 2; ATP binding cassette sub family F (GCN20) member 2; DKFZp586K1823; Drr3; E430001O06; EST133090; fb04f06; fc74f03; fd58d07; HUSSY 18; MABC1; MGC128735; ABCF2_HUMAN. |
研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 轉(zhuǎn)運(yùn)蛋白 表觀遺傳學(xué) |
抗體來(lái)源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 71kDa |
細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ABCF2: 301-400/623 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This protein is a member of the GCN20 subfamily. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]. Similarity: Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily. Contains 2 ABC transporter domains. SWISS: Q9UG63 Gene ID: 10061 Database links: Entrez Gene: 10061 Human Entrez Gene: 27407 Mouse Omim: 612510 Human SwissProt: Q9UG63 Human SwissProt: Q99LE6 Mouse Unigene: 654958 Human Unigene: 21629 Mouse Unigene: 25523 Rat |
產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with ABCF2 polyclonal antibody, unconjugated (bs-12441R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABCF2) Polyclonal Antibody, Unconjugated (bs-12441R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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