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Rabbit Anti-GEM  antibody (bs-13331R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號(hào) bs-13331R
英文名稱 Rabbit Anti-GEM  antibody
中文名稱 GTP結(jié)合絲裂原誘導(dǎo)T細(xì)胞蛋白抗體
別    名 GTP binding mitogen induced T cell protein; GTP binding protein expressed in mitogen stimulated T cells; GTP binding protein GEM; GTP binding protein overexpressed in skeletal muscle; Kinase inducible Ras like protein; KIR; MGC26294; RAS like protein KIR; GEM_HUMAN.  
研究領(lǐng)域 細(xì)胞生物  信號(hào)轉(zhuǎn)導(dǎo)  G蛋白偶聯(lián)受體  淋巴細(xì)胞  t-淋巴細(xì)胞  G蛋白信號(hào)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human (predicted: Mouse,Rat,Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 34kDa
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GEM: 201-296/296 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Gem belongs to the Rad/Gem/Kir (RGK) subfamily of Ras-related GTPases, which lack typical C-terminal amino acid motifs for isoprenylation. Rad and Gem bind calmodulin in a Ca2+-dependent manner via this C-terminal extension, involving residues 278–297 in human Rad. High intracellular Gem levels, which interact with intact microtubules and microfilaments, promote profound changes in cell morphology. Ectopic Gem expression is sufficient to stimulate cell flattening and neurite extension in N1E-115 and SH-SY5Y neuroblastoma cells, suggesting a role for Gem in cytoskeletal rearrangement and/or morphological differentiation of neurons. Gem was also observed in developing trigeminal nerve ganglia in 12.5 day mouse embryos, demonstrating that Gem expression is a property of normal ganglionic development. The interaction of Gem with beta-subunits regulates Ca2+ channel expression at the cell surface. The human Gem gene maps to chromosome ?8q22.1.

Function:
GEM belongs to the RAD/GEM family of GTP-binding proteins. It could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. GEM has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.

Subunit:
Interacts with calmodulin in a Ca(2+)-dependent manner. Binds ROCK1.

Subcellular Location:
Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue Specificity:
Most abundant in thymus, spleen, kidney, lung, and testis. Less abundant in heart, brain, liver and skeletal muscle.

Post-translational modifications:
Phosphorylated on tyrosine residues.

Similarity:
Belongs to the small GTPase superfamily. RGK family.

SWISS:
P55040

Gene ID:
2669

Database links:

Entrez Gene: 2669 Human

Entrez Gene: 14579 Mouse

Entrez Gene: 297902 Rat

Omim: 600164 Human

SwissProt: P55040 Human

SwissProt: P55041 Mouse

Unigene: 654463 Human

Unigene: 247486 Mouse

Unigene: 40436 Rat



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GEM) Polyclonal Antibody, Unconjugated (bs-13331R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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