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Rabbit Anti-CREB5  antibody (bs-14053R)  
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產(chǎn)品編號(hào) bs-14053R
英文名稱(chēng) Rabbit Anti-CREB5  antibody
中文名稱(chēng) 環(huán)腺苷酸應(yīng)答元件結(jié)合蛋白5抗體
別    名 cAMP response element binding protein 5; cAMP response element binding protein CRE Bpa; cAMP response element-binding protein CREBPa; cAMP responsive element binding protein 5; cAMP responsive element binding protein 5 isoform alpha; cAMP-responsive element-binding protein 5; CRE BPA; CRE-BPa; CREB 5; CREB-5; CREB5; CREB5_HUMAN; CREBPA; cyclic AMP responsive element binding protein 5; Cyclic AMP-responsive element-binding protein 5.  
研究領(lǐng)域 細(xì)胞生物  染色質(zhì)和核信號(hào)  信號(hào)轉(zhuǎn)導(dǎo)  轉(zhuǎn)錄調(diào)節(jié)因子  鋅指蛋白  表觀遺傳學(xué)  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Cow,Chicken,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 57kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CREB5: 21-120/508 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The product of this gene belongs to the CRE (cAMP response element)-binding protein family. Members of this family contain zinc-finger and bZIP DNA-binding domains. The encoded protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Function:
Binds to the cAMP response element and activates transcription.

Subunit:
Binds DNA as a homodimer or as a heterodimer with JUN or ATF2/CREBP1.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.

SWISS:
Q02930

Gene ID:
9586

Database links:

Entrez Gene: 9586 Human

Entrez Gene: 231991 Mouse

Entrez Gene: 500131 Rat

SwissProt: Q02930 Human

SwissProt: Q8K1L0 Mouse

Unigene: 437075 Human

Unigene: 321138 Mouse

Unigene: 210235 Rat



產(chǎn)品圖片
Sample: Lane 1: Human HeLa cell lysates Lane 2: Human HepG2 cell lysates Lane 3: Human MCF-7 cell lysates Primary: Anti-CREB5 (bs-14053R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 60 kDa
Paraformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CREB5 ) Polyclonal Antibody, Unconjugated (bs-14053R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CREB5) Polyclonal Antibody, Unconjugated (bs-14053R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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