| 產(chǎn)品編號 | bs-14224R |
| 英文名稱 | Rabbit Anti-DDX28 antibody |
| 中文名稱 | ATP依賴RNA解旋酶DDX28抗體 |
| 別 名 | DDX 28; DDX28; DDX28_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 28; DEAD box polypeptide 28; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 28; FLJ11282; MDDX 28; MDDX28; Mitochondrial DEAD box protein 28. |
| 研究領(lǐng)域 | 細胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 結(jié)合蛋白 線粒體 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Sheep,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60kDa |
| 細胞定位 | 細胞核 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DDX28: 451-540/540 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus. [provided by RefSeq, Jul 2008] Function: May be involved in RNA processing or transport. Has RNA and Mg(2+)-dependent ATPase activity. Subcellular Location: Nucleus. Mitochondrion. Transported between these two compartments. Nuclear localization depends on active RNA polymerase II transcription. Tissue Specificity: Expressed in all tissues tested, including brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, leukocytes, colon, small intestine, ovary and prostate. Similarity: Belongs to the DEAD box helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. SWISS: Q9NUL7 Gene ID: 55794 Database links: Entrez Gene: 55794 Human Omim: 607618 Human SwissProt: Q9NUL7 Human nigene: 458313 Human |
| 產(chǎn)品圖片 |
Sample: testis (Mouse) Lysate at 40 ug
Primary: Anti-DDX28(bs-14224R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-DDX28 Polyclonal Antibody, Unconjugated(bs-14224R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
