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Rabbit Anti-EFCAB4B  antibody (bs-14512R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號(hào) bs-14512R
英文名稱 Rabbit Anti-EFCAB4B  antibody
中文名稱 EFCAB4B蛋白抗體
別    名 EFCAB-4B; Calcium release-activated calcium channel regulator 2A; CRAC channel regulator 2A; CRACR2A; EF hand calcium binding domain 4B; EF hand calcium binding domain containing protein 4B; EF-hand calcium-binding domain-containing protein 4B; EFC4B_HUMAN; EFCAB 4B; Efcab4b; FLJ33805; MGC4266.  
研究領(lǐng)域 細(xì)胞生物  信號(hào)轉(zhuǎn)導(dǎo)  轉(zhuǎn)運(yùn)蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse (predicted: Human,Rat,Rabbit,GuineaPig,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human EFCAB4B: 201-300/1503 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 EF-CAB4B is a 395 amino acid protein belonging to the EF-CAB4 family. Localizing to cytoplasm, EF-CAB4B contains two EF-hand domains and exists as two alternatively spliced isoforms. At low Ca2+ concentrations, EF-CAB4B acts as a calcium-sensor, facilitating the clustering of Orai1 and Stim1 at the junctional regions between plasma membrane and endoplasmic reticulum, leading to regulation of CRAC channel activation. The gene encoding EF-CAB4B maps to human chromosome 12p13.32. Encoding over 1,100 genes within 132 million bases, chromosome 12 makes up about 4.5% of the human genome. A number of skeletal deformities are linked to chromosome 12 including hypochondrogenesis, achondrogenesis and Kniest dysplasia.

Function:
Ca(2+)-binding protein that plays a key role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex.

Subcellular Location:
Cytoplasm.

Similarity:
Belongs to the EFCAB4 family.
Contains 2 EF-hand domains.

SWISS:
Q9BSW2

Gene ID:
74766

Database links:

Entrez Gene: 84766 Human

Entrez Gene: 381812 Mouse

Omim: 614178 Human

SwissProt: Q9BSW2 Human

SwissProt: Q3UP38 Mouse

Unigene: 504534 Human

Unigene: 296093 Mouse



產(chǎn)品圖片
Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-EFCAB4B (bs-14512R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46/83 kD Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EFCAB4B) Polyclonal Antibody, Unconjugated (bs-14512R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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