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Rabbit Anti-ELP4  antibody (bs-14574R)  
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產(chǎn)品編號(hào) bs-14574R
英文名稱(chēng) Rabbit Anti-ELP4  antibody
中文名稱(chēng) ELP4蛋白抗體
別    名 C11orf19; dJ68P15A.1; elongation protein 4 homolog (S. cerevisiae); Elongation protein 4 homolog; Elongator complex protein 4; ELP4; ELP4_HUMAN; FLJ20498; hELP4; PAX6 neighbor gene protein; PAX6NEB; PAXNEB.  
研究領(lǐng)域 細(xì)胞生物  神經(jīng)生物學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  轉(zhuǎn)錄調(diào)節(jié)因子  表觀遺傳學(xué)  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 47kDa
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ELP4: 321-424/424 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a membrane-bound protein which is a member of the ELO family, proteins which participate in the biosynthesis of fatty acids. Consistent with the expression of the encoded protein in photoreceptor cells of the retina, mutations and small deletions in this gene are associated with Stargardt-like macular dystrophy (STGD3) and autosomal dominant Stargardt-like macular dystrophy (ADMD), also referred to as autosomal dominant atrophic macular degeneration. [provided by RefSeq, Jul 2008]

Function:
Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Widely expressed

Similarity:
Belongs to the ELP4 family.

SWISS:
Q96EB1

Gene ID:
26610

Database links:

Entrez Gene: 26610 Human

Entrez Gene: 77766 Mouse

Entrez Gene: 100514702 Pig

Entrez Gene: 691133 Rat

Entrez Gene: 550331 Zebrafish

Omim: 606985 Human

SwissProt: Q2TBH6 Cow

SwissProt: Q96EB1 Human

SwissProt: Q9ER73 Mouse

SwissProt: Q566Y1 Zebrafish

Unigene: 175534 Human

Unigene: 33870 Mouse

Unigene: 79765 Zebrafish



產(chǎn)品圖片
Protein: Hela(human) lysate at 40ug; Primary: rabbit Anti-ELP4 (bs-14574R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 47 kD Observed band size: 47 kD
Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Prostate tissue lysates Lane 3: Rat Stomach tissue lysates Lane 4: Rat Cerebrum tissue lysates Primary: Anti-ELP4 (bs-14574R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ELP4) Polyclonal Antibody, Unconjugated (bs-14574R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ELP4) Polyclonal Antibody, Unconjugated (bs-14574R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ELP4) Polyclonal Antibody, Unconjugated (bs-14574R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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