| 產(chǎn)品編號 | bs-16514R |
| 英文名稱 | Rabbit Anti-ZNF687 antibody |
| 中文名稱 | 鋅指蛋白687抗體 |
| 別 名 | 4931408L03Rik; DKFZp781I1719; KIAA1441; mKIAA1441; RP11-126K1.3; Zfp687; Zinc finger protein 687; ZN687_HUMAN; ZNF687. |
| 研究領(lǐng)域 | 轉(zhuǎn)錄調(diào)節(jié)因子 鋅指蛋白 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 129kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF687: 1001-1100/1237 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a kruppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF687 (zinc finger protein 687) is a 1,237 amino acid nuclear protein that is involved in transcriptional regulation. A member of the Krüppel C2H2-type zinc-finger protein family, ZNF687 contains ten C2H2-type zinc fingers and exists as two alternatively spliced isoforms. The gene encoding ZNF687 maps to human chromosome 1, which comprises nearly 8% of the human genome and houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. Function: May be involved in transcriptional regulation. Subcellular Location: Nucleus. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 10 C2H2-type zinc fingers. SWISS: Q8N1G0 Gene ID: 57592 Database links: Entrez Gene: 57592 Human Entrez Gene: 78266 Mouse Omim: 610568 Human SwissProt: Q8N1G0 Human SwissProt: Q9D2D7 Mouse Unigene: 186756 Human Unigene: 389478 Mouse |
| 產(chǎn)品圖片 |
Sample:
A549(Human) Cell Lysate at 40 ug
U251(Human) Cell Lysate at 40 ug
Primary: Anti-ZNF687 (bs-16514R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129 kD
Observed band size: 129 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF687) Polyclonal Antibody, Unconjugated (bs-16514R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
