| 產(chǎn)品編號 | bs-17198R |
| 英文名稱 | Rabbit Anti-JAML antibody |
| 中文名稱 | 粘附分子JAML蛋白抗體 |
| 別 名 | adhesion molecule AMICA; Adhesion molecule interacting with CXADR antigen 1; adhesion molecule interacts with CXADR antigen 1; adhesion molecule that interacts with CXADR antigen 1; AMICA; Amica1; CREA7-1; CREA7-4; Dendritic cell-specific protein CREA7-1; dendritic-cell specific protein CREA7-1; dendritic-cell specific protein CREA7-4; FLJ37080; Gm638; JAML1_HUMAN; junctional adhesion molecule; Junctional adhesion molecule-like; Junctional adhesion molecule-like precursor; MGC118814; MGC118815; UNQ722/PRO1387. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 細胞粘附分子 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human JAML: 151-250/394 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
JAML is a 394 amino acid single-pass type I membrane protein and novel adhesion molecule with localization at the cell plasma membrane in regions of cell-to-cell contact but not free cell borders, implying that JAML participates in homophilic interactions. JAML belongs to the immunoglobulin superfamily and is expressed in peripheral blood leukocytes, bone marrow, spleen, lymph nodes and fetal thymus, liver and spleen. JAML assists in the migration of leukocytes through endothelilal and epithelial tissues, mediates binding with CAR (coxsackie and adenovirus receptor) via its Ig-like V-type domain 2, and strengthens the binding of leukocytes to endothelial cells. Consisting of a transmembrane segment, cytoplasmic tail, 19-amino acid signal peptide and two extracellular immunoglobulin-like domains, JAML also undergoes alternative splicing resulting in three isoforms and is encoded by a gene mapping to human chromosome 11q23.3. Function: May function in transmigration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, it mediates adhesive interactions with CXADR, a protein of the junctional complex of epithelial cells. Enhances myeloid leukemia cell adhesion to endothelial cells. Subcellular Location: Cell junction. Cell membrane. Localized at the plasma membrane in areas of cell-cell contacts and at the cell surface of PMN. Tissue Specificity: Expression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus. Similarity: Belongs to the immunoglobulin superfamily. Contains 2 Ig-like V-type (immunoglobulin-like) domains. SWISS: Q86YT9 Gene ID: 120425 Database links: Entrez Gene: 120425 Human Entrez Gene: 270152 Mouse Omim: *609770 Human SwissProt: Q86YT9 Human SwissProt: Q80UL9 Mouse Unigene: 16291 Human Unigene: 190461 Mouse Unigene: 21010 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
