產(chǎn)品編號 | bs-17687R |
英文名稱 | Rabbit Anti-MPP6/MPHOSPH6 antibody |
中文名稱 | M-期磷蛋白6抗體 |
別 名 | M phase phosphoprotein 6; M phase phosphoprotein 6 variant; M-phase phosphoprotein 6; MPH6_HUMAN; MPHOSH6; MPHOSPH 6; Mphosph6; MPP-6; MPP; MPP6. |
研究領(lǐng)域 | 細胞生物 細胞粘附分子 表觀遺傳學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Sheep,Cow) |
產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 19kDa |
細胞定位 | 細胞核 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human MPP6: 1-80/160 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
MPHOSPH6 (M-Phase Phosphoprotein 6) is a Protein Coding gene. Among its related pathways are rRNA processing in the nucleus and cytosol and Gene Expression. Gene Ontology (GO) annotations related to this gene include RNA binding. Function: RNA-binding protein that associates with the RNA exosome complex. Involved in the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA and play a role in recruiting the RNA exosome complex to pre-rRNA; this function may include C1D. Subunit: Associates with the RNA exosome complex, probably mediated by EXOSC10. Interacts with ARHGAP18, EXOSC10 and SKIV2L2. Subcellular Location: Nucleus > nucleolus. Cytoplasm. Cytoplasmic in M phase. Post-translational modifications: Phosphorylated in M (mitotic) phase. Similarity: Belongs to the MPP6 family. SWISS: Q99547 Gene ID: 10200 Database links: Entrez Gene: 10200 Human Entrez Gene: 68533 Mouse Omim: 605500 Human SwissProt: Q99547 Human SwissProt: Q9D1Q1 Mouse Unigene: 344400 Human Unigene: 181836 Mouse |
產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP6/MPHOSPH6) Polyclonal Antibody, Unconjugated (bs-17687R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat urinary bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP6/MPHOSPH6) Polyclonal Antibody, Unconjugated (bs-17687R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |