| 產品編號 | bs-18172R |
| 英文名稱 | Rabbit Anti-ISX/Intestine specific homeobox antibody |
| 中文名稱 | 胰腸同源結構域轉錄因子抗體 |
| 別 名 | DKFZp781N2395; Intestine-specific homeobox; Isx; ISX_HUMAN; MGC138417; OTTHUMP00000028558; Pancreas intestine homeodomain transcription factor; Pix 1; RAX like homeobox; RAX-like homeobox; RAXLX. |
| 研究領域 | 細胞生物 轉錄調節(jié)因子 轉運蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Sheep,Cow,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ISX/Intestine specific homeobox: 81-180/245 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Homeobox genes encode DNA-binding proteins, many of which are thought to be involved in early embryonic development. Homeobox genes encode a DNA-binding domain of 60 to 63 amino acids referred to as the homeodomain. This gene is a member of the RAXLX homeobox gene family. [provided by RefSeq, Jul 2008] Function: Transcription factor that regulates gene expression in intestine. May participate in vitamin A metabolism most likely by regulating BCMO1 expression in the intestine. Subcellular Location: Nucleus. Similarity: Contains 1 homeobox DNA-binding domain. SWISS: Q2M1V0 Gene ID: 91464 Database links: Entrez Gene: 91464 Human Omim: 612019 Human SwissProt: Q2M1V0 Human Unigene: 567637 Human |
| 產品圖片 |
Sample:
Lane 1: Lovo (Human) Cell Lysate at 30 ug
Lane 2: SW480 (Human) Cell Lysate at 30 ug
Primary:
Anti-ISX/Intestine specific homeobox (bs-18172R) at 1:500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
Sample:
small intestine (Mouse) Lysate at 40 ug
large intestine (Mouse) Lysate at 40 ug
Primary: Anti-ISX/Intestine specific homeobox (bs-18172R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ISX) Polyclonal Antibody, Unconjugated (bs-18172R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
