| 產(chǎn)品編號 | bs-13455R |
| 英文名稱 | Rabbit Anti-Fibronectin/Ugl-Y3 antibody |
| 中文名稱 | 纖維連接蛋白/Ugl-Y3抗體 |
| 別 名 | CIG; Cold insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED B; ED-B; fibronectin 1; FINC; FN 1; FN; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor; MSF; Transformation sensitive protein; FINC_HUMAN; Fibronectin; Cold-insoluble globulin; Ugl-Y3. |
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Specific References (3) | bs-13455R has been referenced in 3 publications.
[IF=9.038] Bing Han. et al. Pulmonary inflammatory and fibrogenic response induced by graphitized multi-walled carbon nanotube involved in cGAS-STING signaling pathway. J Hazard Mater. 2021 May;:125984 WB ; Mouse.
[IF=6.978] Atsushi Tamura. et al. Supramolecular Surface Coatings with Acetylated Polyrotaxane-Based Triblock Copolymers for Thermal Regulation of Cell Adhesion and Fabrication of Cell Sheets. BIOMACROMOLECULES. 2022;XXXX(XXX):XXX-XXX IHC, IF ; Mouse.
[IF=5.295] Yue-hua Liu. et al. ANKRD49 promotes the invasion and metastasis of lung adenocarcinoma via a P38/ATF-2 signalling pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2022 Jun 30 IHC ; Human.
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| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 細胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ICC/IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 259kDa |
| 細胞定位 | 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Fibronectin/Ugl-Y3: 2201-2300/2386 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]. Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Subunit: Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. Interacts with FBLN7. Interacts with COMP. Interacts with S.aureus fnbA. Interacts with TNR; the interaction inhibits cell adhesion and neurite outgrowth. Interacts with FST3. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. Post-translational modifications: Sulfated. It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylation sites are present in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. DISEASE: Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2) [MIM:601894]; also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Similarity: Contains 12 fibronectin type-I domains. Contains 2 fibronectin type-II domains. Contains 16 fibronectin type-III domains. SWISS: P02751 Gene ID: 2335 Database links: Entrez Gene: 2335 Human Entrez Gene: 14268 Mouse Omim: 135600 Human SwissProt: P02751 Human SwissProt: P11276 Mouse |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Kidney (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Rat) Lysate at 40 ug
Primary: Anti-Fibronectin/Ugl-Y3 (bs-13455R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 263 kD
Observed band size: 263 kD
Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fibronectin/Ugl-Y3) Polyclonal Antibody, Unconjugated (bs-13455R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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