| 產(chǎn)品編號 | bs-19536R |
| 英文名稱 | Rabbit Anti-ZNF502 antibody |
| 中文名稱 | 鋅指蛋白502抗體 |
| 別 名 | FLJ12515; FLJ14855; Zinc finger protein 502. |
| 研究領域 | 轉錄調節(jié)因子 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF502: 5-150/544 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ZNF502 may be involved in transcriptional regulation. It belongs to the krueppel C2H2 type zinc finger protein family. ZNF502 contains fourteen C2H2 type zinc fingers. Function: May be involved in transcriptional regulation. Subcellular Location: Nucleus Similarity: Contains 14 C2H2-type zinc fingers. SWISS: Q8TBZ5 Gene ID: 91392 Database links: Entrez Gene: 91392 Human SwissProt: Q8TBZ5 Human Unigene: 224843 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human U-2 OS cell lysates
Lane 3: Human HUVEC cell lysates
Lane 4: Human 293T cell lysates
Lane 5: Human A549 cell lysates
Primary: Anti-ZNF502 (bs-19536R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 60 kDa
Tissue/cell: human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-ZNF502 Polyclonal Antibody, Unconjugated(bs-19536R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
