產(chǎn)品編號 | bs-19640R |
英文名稱 | Rabbit Anti-OLFML2B antibody |
中文名稱 | 嗅介蛋白樣蛋白2B抗體 |
別 名 | Olfactomedin like 2B; Olfactomedin like protein 2B; Olfactomedin-like protein 2B; OLFML2B; OLM2B_HUMAN; Photomedin 2; Photomedin-2; RP11-227F8.1. |
Specific References (1) | bs-19640R has been referenced in 1 publications.
[IF=3.288] Liu J et al. Bioinformatic exploration of OLFML2B overexpression in gastric cancer base on multiple analyzing tools.BMC?Cancer.?2019 Mar 13;19(1):227. IHC-P ; Human.
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研究領(lǐng)域 | 細胞生物 神經(jīng)生物學 干細胞 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human,Mouse (predicted: Cow) |
產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 84kDa |
細胞定位 | 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human OLFML2B: 451-550/750 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 | |
產(chǎn)品圖片 |
Sample:
Eye (Mouse) Lysate at 40 ug
Epithelium (Mouse) Lysate at 40 ug
Primary: Anti-OLFML2B(bs-19640R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84kD
Observed band size: 84kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OLFML2B) Polyclonal Antibody, Unconjugated (bs-19640R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OLFML2B) Polyclonal Antibody, Unconjugated (bs-19640R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |