產(chǎn)品編號 | bs-19812R |
英文名稱 | Rabbit Anti-SLC34A1 antibody |
中文名稱 | 溶質(zhì)載體家族蛋白34成員A1抗體 |
別 名 | FRTS2; Na(+)-dependent phosphate cotransporter 2A; Na(+)/Pi cotransporter 2A; Na+-phosphate cotransporter type II; naPi-2a; NAPI-3; naPi2a; NAPI3; NPHLOP1; NPT2; NPTIIa; renal sodium-dependent phosphate transporter; renal sodium/phosphate transporter protein 2; SLC11; SLC17A2; SLC17A2, formerly; NPT2A_HUMAN; sodium-dependent phosphate transport protein 2A; sodium-phosphate transport protein 2A; sodium/phosphate co-transporter; sodium/phosphate cotransporter 2A; sodium/phosphate transporter 2, kidney; solute carrier family 17 (sodium phosphate), member 2; solute carrier family 17, member 2, formerly; solute carrier family 34 (sodium phosphate), member 1; solute carrier family 34 (type II sodium/phosphate. |
研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 轉(zhuǎn)運蛋白 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cat) |
產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 69kDa |
細胞定位 | 細胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SLC34A1: 101-200/639 <Extracellular> |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
This gene encodes a member of the type II sodium-phosphate cotransporter family. Mutations in this gene are associated with hypophosphatemia nephrolithiasis/osteoporosis 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009] Function: May be involved in actively transporting phosphate into cells via Na+ cotransport in the renal brush border membrane. Probably mediates 70-80% of the apical influx. Subunit: Interacts via its C-terminal region with PDZK2 (By similarity). Interacts with SLC9A3R1. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Kidney and lung. DISEASE: The disease is caused by mutations affecting the gene represented in this entry. Disease description:A disease characterized by decreased renal phosphate absorption, renal phosphate wasting, hypophosphatemia, hyperphosphaturia, hypercalciuria, nephrolithiasis and osteoporosis. Similarity: Belongs to the SLC34A transporter family. SWISS: Q06495 Gene ID: 6569 Database links: Entrez Gene: 100735387 Guinea pig Entrez Gene: 100058397 Horse Entrez Gene: 6569 Human Entrez Gene: 20505 Mouse Entrez Gene: 100009392 Rabbit Entrez Gene: 443505 Sheep Omim: 182309 Human SwissProt: Q06495 Human SwissProt: Q60825 Mouse SwissProt: Q28620 Rabbit SwissProt: O97704 Sheep Unigene: 936 Human Unigene: 276355 Mouse Unigene: 92022 Rat |
產(chǎn)品圖片 |
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SLC34A1 Polyclonal Antibody, Unconjugated(bs-19812R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SLC34A1 Polyclonal Antibody, Unconjugated(bs-19812R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |