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Rabbit Anti-SLIRP/C14orf156  antibody (bs-21033R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-21033R
英文名稱 Rabbit Anti-SLIRP/C14orf156  antibody
中文名稱 14號染色體開放閱讀框156抗體
別    名 C14orf156; Chromosome 14 open reading frame 156; DC50; DC23; PD04872; SLIRP; SRA stem loop interacting RNA binding protein, mitochondrial.  
研究領(lǐng)域 細(xì)胞生物  信號轉(zhuǎn)導(dǎo)  生長因子和激素  結(jié)合蛋白  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit,Pig)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 12kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SLIRP/C14orf156: 31-100/109 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Steroid receptor RNA activator (SRA, or SRA1; MIM 603819) is a complex RNA molecule containing multiple stable stem-loop structures that functions in coactivation of nuclear receptors. SLIRP interacts with stem-loop structure-7 of SRA (STR7) and modulates nuclear receptor transactivation (Hatchell et al., 2006 [PubMed 16762838]).[supplied by OMIM, Mar 2008]

Function:
RNA-binding protein that acts as a nuclear receptor corepressor. Probably acts by binding the SRA RNA, and repressing the SRA-mediated nuclear receptor coactivation. Binds the STR7 loop of SRA RNA. Also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation.

Subcellular Location:
Mitochondrial and Nuclear; Predominantly mitochondrial.

Tissue Specificity:
Ubiquitously expressed, with highest level in heart, liver, skeletal muscle and testis.

Similarity:
Contains 1 RRM (RNA recognition motif) domain.

SWISS:
Q9GZT3

Gene ID:
81892

Database links:

Entrez Gene: 81892 Human

Omim: 610211 Human

SwissProt: Q9GZT3 Human

Unigene: 655105 Human



產(chǎn)品圖片
Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti- SLIRP/C14orf156 (bs-21033R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kD Observed band size: 11 kD
Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-C14orf156 (bs-21033R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kD Observed band size: 12 kD
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLIRP/C14orf156) Polyclonal Antibody, Unconjugated (bs-21033R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLIRP/C14orf156) Polyclonal Antibody, Unconjugated (bs-21033R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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