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Rabbit Anti-VPS25  antibody (bs-4472R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-4472R
英文名稱 Rabbit Anti-VPS25  antibody
中文名稱 液泡蛋白分選蛋白25抗體
別    名 Dermal papilla derived protein 9; Dermal papilla-derived protein 9; DERP9; EAP20; ELL associated protein of 20 kDa; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25; FAP20; HGNC:28122; hVps25; MGC10540; Vacuolar protein sorting 25 homolog (S. cerevisiae); Vacuolar protein sorting associated protein 25; Vacuolar protein-sorting-associated protein 25; VPS25; VPS25_HUMAN.  
研究領(lǐng)域 細胞生物  信號轉(zhuǎn)導  淋巴細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Rat (predicted: Human,Mouse,Rabbit,Cow,Dog,Horse)
產(chǎn)品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 21kDa
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human VPS25: 21-120/176 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]

Function:
Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.

Subcellular Location:
Cytoplasm. Endosome membrane. Nucleus > nucleoplasm. Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6.

Tissue Specificity:
Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung.

Similarity:
Belongs to the VPS25 family.

SWISS:
Q9BRG1

Gene ID:
84313

Database links:

Entrez Gene: 84313 Human

Entrez Gene: 28084 Mouse

Omim: 610907 Human

SwissProt: Q9BRG1 Human

SwissProt: Q9CQ80 Mouse

Unigene: 500165 Human

Unigene: 301020 Mouse



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VPS25) Polyclonal Antibody, Unconjugated (bs-4472R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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