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Rabbit Anti-FBP1  antibody (bs-20431R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號(hào) bs-20431R
英文名稱 Rabbit Anti-FBP1  antibody
中文名稱 果糖二磷酸酶1抗體
別    名 6-bisphosphatase 1; 6-bisphosphate 1-phosphohydrolase 1; D fructose 1 6 bisphosphate 1 phosphohydrolase 1; D-fructose-1; EC 3.1.3.11; F16P1_HUMAN; FBP; FBP 1; FBPase 1; Fructose 1 6 bisphosphatase 1; Fructose bisphosphatase 1; Fructose-1; Growth inhibiting protein 17; Liver fructose bisphosphatase.  
研究領(lǐng)域 腫瘤  染色質(zhì)和核信號(hào)  信號(hào)轉(zhuǎn)導(dǎo)  通道蛋白  新陳代謝  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit,Pig,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,Flow-Cyt=1ug/Test,ICC/IF=1:25
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 37kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human FBP1: 51-150/338 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. [provided by RefSeq, Jul 2008]

Subunit:
Homotetramer

Similarity:
Belongs to the FBPase class 1 family.

SWISS:
P09467

Gene ID:
2203

Database links:

Entrez Gene: 2203 Human

Entrez Gene: 14121 Mouse

Entrez Gene: 24362 Rat

Omim: 611570 Human

SwissProt: P09467 Human

SwissProt: Q9QXD6 Mouse

SwissProt: P19112 Rat

Unigene: 494496 Human

Unigene: 423078 Mouse

Unigene: 33703 Rat



產(chǎn)品圖片
Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse Liver tissue lysates Primary: Anti-FBP1 (bs-20431R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 40 kDa
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (FBP1) polyclonal Antibody, Unconjugated (bs-20431R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control(black line):MCF-7. Primary Antibody (green line): Rabbit Anti-FBP1 antibody (bs-20431R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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