| 產(chǎn)品編號 | bs-4454R |
| 英文名稱 | Rabbit Anti-SEC31B antibody |
| 中文名稱 | 轉(zhuǎn)運蛋白SEC31B抗體 |
| 別 名 | SEC31L2; Gm341; Protein transport protein Sec31B; SC31B_HUMAN; SEC31 B; SEC31 homolog B (S. cerevisiae); SEC31 homolog B; SEC31 like 2; SEC31 related protein B; SEC31-like protein 2; SEC31-related protein B; SEC31B 1; SEC31B; SEC31B-1; SEC31L2; Secretory pathway component Sec31B 1; secretory pathway component Sec31B1. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 129kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SEC31B: 481-580/1179 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein of unknown function. The protein has moderate similarity to rat VAP1 protein which is an endosomal membrane-associated protein, containing a putative Ca2+/calmodulin-dependent kinase II phosphorylation site. [provided by RefSeq, Jul 2008] Function: As a component of the coat protein complex II (COPII), may function in vesicle budding and cargo export from the endoplasmic reticulum. Subcellular Location: Cytoplasm. Cytoplasmic vesicle > COPII-coated vesicle membrane. Endoplasmic reticulum membrane. Tissue Specificity: Ubiquitously expressed at low levels with specific expression in thymus and testis. Expressed in testis by Sertoli cells, Leydig cells and spermatogonia and in cerebellum more prominently by Purkinje and granular cells (at protein level). Post-translational modifications: Monoubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex, leading to regulate the size of COPII coats. Similarity: Belongs to the WD repeat SEC31 family. Contains 8 WD repeats. SWISS: Q9NQW1 Gene ID: 25956 Database links: Entrez Gene: 25956 Human Entrez Gene: 240667 Mouse Omim: 610258 Human SwissProt: Q9NQW1 Human SwissProt: Q3TZ89 Mouse Unigene: 18889 Human Unigene: 329873 Mouse |
| 產(chǎn)品圖片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
SY5Y(Human) Cell Lysate at 30 ug
Primary: Anti- SEC31B (bs-4454R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129 kD
Observed band size: 129 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
