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Rabbit Anti-RARS2  antibody (bs-21159R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-21159R
英文名稱 Rabbit Anti-RARS2  antibody
中文名稱 精氨酸t(yī)RNA連接酶2抗體
別    名 arginine tRNA ligase; arginyl tRNA synthetase 2 mitochondrial; Arginyl tRNA synthetase; Arginyl-tRNA synthetase; ArgRS; DALRD2; mitochondrial; PCH6; Probable arginine tRNA ligase; probable arginine tRNA ligase mitochondrial; Probable arginine--tRNA ligase; probable arginyl tRNA synthetase mitochondrial; RARS2; RARSL; SYRM_HUMAN.  
研究領(lǐng)域 發(fā)育生物學(xué)  神經(jīng)生物學(xué)  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 64kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human RARS2: 221-320/578 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene is an arginyl-tRNA synthetase that is found in the mitochondrial matrix. Defects in this gene are a cause of pontocerebellar hypoplasia type 6 (PCH6). [provided by RefSeq, Oct 2008]

Subcellular Location:
Mitochondrion matrix.

Similarity:
Belongs to the class-I aminoacyl-tRNA synthetase family.

SWISS:
Q5T160

Gene ID:
57038

Database links:

Entrez Gene: 525894 Cow

Entrez Gene: 57038 Human

Omim: 611524 Human

SwissProt: Q0P5H7 Cow

SwissProt: Q5T160 Human

Unigene: 16559 Cow

Unigene: 485910 Human



Defects in RARS2 are the cause of pontocerebellar hypoplasia type 6 (PCH6) [MIM:611523]; also known as fatal infantile encephalopathy with mitochondrial respiratory chain defects. Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem.
產(chǎn)品圖片
Sample: HepG2(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti-RARS2 (bs-21159R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD
Sample: Large intestine (Mouse) Lysate at 40 ug Primary: Anti- RARS2 (bs-21159R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 62 kD
Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti- RARS2 (bs-21159R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RARS2) Polyclonal Antibody, Unconjugated (bs-21159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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