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Mouse Anti-HP1 gamma  antibody (bsm-33376M)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bsm-33376M
英文名稱 Mouse Anti-HP1 gamma  antibody
中文名稱 異染色質(zhì)蛋白1-γ/HP-1γ單克隆抗體
別    名 CBX 3; CBX3; Chromobox homolog 3; Chromobox protein homolog 3; HECH; Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma; Heterochromatin protein HP1 gamma; HP1 gamma homolog; HP1Hs gamma ; Modifier 2; protein HP1-GAMMA; HP1g; M32; MGC118084; CBX3_HUMAN; Modifier 2 protein;   HP-1-γ; HP 1γ; HP-1γ; HP-1 γ; HP1γ; HP1 γ;
研究領(lǐng)域 腫瘤  細(xì)胞生物  染色質(zhì)和核信號  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 6F4
交叉反應(yīng) Human,Rat (predicted: Mouse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 21kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human HP1 gamma Protein: full length 
亞    型
純化方法 affinity purified by Protein G
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Function:
Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Subunit:
Binds directly to CHAF1A. Interacts with histone H3 methylated at 'Lys-9'. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2 and SP100. Interacts with TIF1A. Interacts with MIS12 and DSN1. Can interact directly with CBX5 via the chromoshadow domain. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1.

Subcellular Location:
Nucleus (Potential). Note=Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis (Potential).

Post-translational modifications:
Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.

Similarity:
Contains 2 chromo domains.

SWISS:
Q13185

Gene ID:
11335

Database links:

Entrez Gene: 11335 Human

Entrez Gene: 12417 Mouse

Entrez Gene: 297093 Rat

Omim: 604477 Human

SwissProt: Q13185 Human

SwissProt: P23198 Mouse

Unigene: 381189 Human

Unigene: 280968 Mouse

Unigene: 458234 Mouse

Unigene: 485141 Mouse




產(chǎn)品圖片
Sample: Molt-4 cell (human) Lysate at 30 ug jurkat cell (human) Lysate at 30 ug HepG2 cell (human) Lysate at 30 ug Primary: Anti- HP1 gamma(bsm-33376M)at 1/5000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD
Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HP1 gamma) Monoclonal Antibody, Unconjugated (cell supernatant of bsm-33376M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HP1 gamma) Monoclonal Antibody, Unconjugated (cell supernatant of bsm-33376M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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