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BiossECL Plus WB Substrate (C05-07004)  
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說明書: 下載
12.5ml×2/280.00元
50ml×2/880.00元
250ml×2/3200.00元
大包裝/詢價
產(chǎn)品編號 C05-07004
英文名稱 BiossECL Plus WB Substrate
中文名稱 BiossECL超敏發(fā)光液
別    名 ECL Western Blotting Substrate; BiossECL Plus Western Blotting Substrate Kit; ECL Western Blotting Substrate Kit; Ultrasensitive ECL Kit; BiossECL Plus; Western Blotting Luminol Reagent;   極超敏ECL化學發(fā)光試劑盒; UltraSignal 超敏ECL化學發(fā)光底物;UltraSignal 超敏ECL化學發(fā)光液; ECL Plus超敏發(fā)光液; ECL超敏發(fā)光液; ECL超敏發(fā)光底物; ECL發(fā)光液; ECL Plus級發(fā)光液; 超敏化學發(fā)光檢測試劑盒; Western螢光檢測試劑盒; 魯米諾化學發(fā)光試劑; 增強型ECL 化學發(fā)光檢測試劑盒(即用型);
Specific References  (16)     |     C05-07004 has been referenced in 16 publications.
[IF=17.694] Guo, Yuxin. et al. In situ generation of micrometer-sized tumor cell-derived vesicles as autologous cancer vaccines for boosting systemic immune responses. NAT COMMUN. 2022 Nov;13(1):1-20  
[IF=10.317] Zhuo Wang. et al. Targeted galectin-7 inhibition with ultrasound microbubble targeted gene therapy as a sole therapy to prevent acute rejection following heart transplantation in a Rodent model. Biomaterials. 2020 Dec;263:120366  
[IF=6.792] Li Z et al. Polystyrene microplastics cause cardiac fibrosis by activating Wnt/β-catenin signaling pathway and promoting cardiomyocyte apoptosis in rats. Environ Pollut . 2020 Oct;265(Pt A):115025.  WB ;  
[IF=4.872] Junyu Hou. et al. Polystyrene microplastics lead to pyroptosis and apoptosis of ovarian granulosa cells via NLRP3/Caspase-1 signaling pathway in rats. Ecotox Environ Safe. 2021 Apr;212:112012  other ;  
[IF=3.848] Zuo Qiang. et al. Targeting GRP78 enhances the sensitivity of HOS osteosarcoma cells to pyropheophorbide-α methyl ester-mediated photodynamic therapy via the Wnt/β-catenin signaling pathway. Acta Bioch Bioph Sin. 2021 Sep;:  WB ;  human .  
[IF=3.654] Mengying Zhang. et al. Preparation of Black pepper (Piper nigrum L.) essential oil nanoparticles and its antitumor activity on triple negative breast cancer in vitro. J FOOD BIOCHEM. 2022 Sep;:e14406  Other ;  Other.  
[IF=3.647] Zhang C et al. Co-delivery of EGFR and BRD4 siRNA by cell-penetrating peptides-modified redox-responsive complex in triple negative breast cancer cellsLife Sci.2020 Dec 10;266:118886.  WB ;  
[IF=3.34] Zhang, Yi-Dan. et al. Role of synaptotagmin 13 (SYT13) in promoting breast cancer and signaling pathways. CLIN TRANSL ONCOL. 2023 Jan;:1-12  
[IF=3.298] Zhongxiao Zhou. et al. Formononetin regulates endothelial nitric oxide synthase to protect vascular endothelium in deep vein thrombosis rats:. INT J IMMUNOPATH PH. 2022;():  
[IF=3.056] Shengda Li. et al. Polystyrene microplastics induce blood–testis barrier disruption regulated by the MAPK-Nrf2 signaling pathway in rats. 2021 Apr 25  Other ;  
[IF=2.952] Guanglei Han. et al. Salvianolic acid?B acts against non?small cell lung cancer A549 cells via inactivation of the MAPK and Smad2/3 signaling pathways. Mol Med Rep. 2022 May;25(5):1-11  Other ;  Other.  
[IF=2.903] Fangming Guo. et al. Shenfu Administration Improves Cardiac Fibrosis in Rats With Myocardial Ischemia-Reperfusion Through Adenosine A2a Receptor Activation:. Hum Exp Toxicol. 2022;():  Other ;  Other.  
[IF=2.745] Zhonghua An. et al. Chamomile Essential Oil: chemical constituents and antitumor activity in MDA-MB-231 cells through PI3K/Akt/mTOR signaling pathway. CHEM BIODIVERS. 2023 Mar;:e202200523  
[IF=2.65] Sitong Ming. et al. Protective Effect of Shengmaiyin in Myocardial Hypertrophy-Induced Rats: A Genomic Analysis by 16S rDNA. EVID-BASED COMPL ALT. 2022 Sep 07;2022:3188292  Other ;  Other.  
[IF=2.315] Lin Chen. et al. Preparation, structural characterization and neuroprotective effects of polysaccharides from the pericarp of Zanthoxylum bungeanum Maxim against H2O2-induced oxidative damage in PC12 cells. S Afr J Bot. 2021 Nov;142:165  WB ;  Rat.  
[IF=2.089] Zhongxue Ye. et al. DNMT3B attenuated the inhibition of TET3 on epithelial-mesenchymal transition in TGF-β1-induced ovarian cancer by methylating the TET3 promoter. REPROD BIOL. 2022 Dec;22:100701  Other ;  Other.  
保存條件 Store at 2-8℃. Protect from light for one year.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 簡介
BiossECL超敏發(fā)光液直接或間接檢測與辣根過氧化物酶HRP關聯(lián)的蛋白或核酸底物。這一獨特的發(fā)光底物系統(tǒng)是目前最靈敏的商業(yè)化熒光ECL檢測試劑,具有極高靈敏度和高信噪比,可檢測出10-100 fg的微量抗原;發(fā)光迅速,熒光可使X 感光膠片感光達12小時以上,特別適用于痕量蛋白或核酸檢測。同時該試劑允許使用更高的抗體稀釋倍數(shù)(1:2000-1:10000),極其節(jié)省抗體。

試劑盒內(nèi)容
25ml規(guī)格:A液12.5ml + B液12.5ml
100ml規(guī)格:A液50ml + B液50ml
500ml規(guī)格:A液250ml + B液250ml

顯色步驟
1、執(zhí)行常規(guī)SDS-PAGE、轉(zhuǎn)膜和Western Blot封閉、一抗孵育、二抗孵育操作。
2、二抗適用HRP標記二抗,適用恰當?shù)南♂尡壤?br /> 3、制備ECL工作液:A液和B液等體積的混合,例如1ml A液和1ml B液混合,混勻后即可使用。用量以充分覆蓋印跡膜為基準,每10 cm2膜需要大約1 ml工作液。
4、將印跡膜有蛋白的一面朝上平整的鋪在一張平板上,加上配好的發(fā)光底物工作液。
5、將工作液均勻滴加在印跡膜上,孵育1-5min??蓪⒁粷崈舻谋ur膜或透明的玻璃紙平整的鋪在孵育有工作液的印跡膜上,輕輕趕出其間的氣泡。
6、在暗室中取一張X膠片小心置于膜上,曝光5秒至1分鐘,立即顯影定影。
7、根據(jù)其發(fā)光的強度,縮短或延長X片的曝光時間(對微弱信號,曝光時間可延長至數(shù)小時),或者曝光一系列不同的時間后再顯影定影挑選一張滿意的。也可用合適的照相器材直接記錄蛋白膜的化學發(fā)光圖像。

安全性:
無特殊毒性,按普通化學品處理。如果不慎與眼、皮膚和衣物接觸,請立刻用大量清水沖洗。

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