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RIPA Lysis Buffer (C05-01001)  
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說明書: 100ml  
100ml/200.00元
大包裝/詢價
產(chǎn)品編號 C05-01001
英文名稱 RIPA Lysis Buffer
中文名稱 RIPA裂解液(W)/RIPA裂解液(弱)
別    名 Radio Immunoprecipitation Assay Lysis Buffer; RIPA Lysis Buffer, Medium; RIPA Lysis Buffer (Medium); Medium; RIPA Lysis Buffer (M); Medium?RIPA?Lysis?Buffer; Mammalian Total Protein Extraction Kit; Protein Extraction Kit;  細(xì)胞裂解液; 組織細(xì)胞裂解液; 蛋白提取液; RIPA裂解液(弱);
Specific References  (19)     |     C05-01001 has been referenced in 19 publications.
[IF=6.638] Wang Z et al. Light-activatable?dual?prodrug?polymer?nanoparticle?for?precise?synergistic?chemotherapy?guidedby?drug-mediated?computed?tomography?imaging. Acta Biomater.?2019 May 22.  Other ;  
[IF=6.064] Tianyu Zhang. et al. Different Extracellular β-Amyloid (1-42) Aggregates Differentially Impair Neural Cell Adhesion and Neurite Outgrowth through Differential Induction of Scaffold Palladin. BIOMOLECULES. 2022 Dec;12(12):1808  
[IF=5.878] Hong-He Xiao. et al. Icarisid II rescues cognitive dysfunction via activation of Wnt/β-catenin signaling pathway promoting hippocampal neurogenesis in APP/PS1 transgenic mice. 2022 Mar 01  Other ;  Other.  
[IF=5.572] Jiayuan Luo. et al. Effects of saponins isolated from Polygonatum sibiricum on H2O2-induced oxidative damage in RIN-m5F cells and its protective effect on pancreas. FOOD CHEM TOXICOL. 2023 May;175:113724  
[IF=5.443] Li Wenjing. et al. CGF Membrane Promotes Periodontal Tissue Regeneration Mediated by hUCMSCs through Upregulating TAZ and Osteogenic Differentiation Genes. Stem Cells Int. 2021;2021:6644366  WB ;  Human.  
[IF=4.757] Yunna Zhang. et al. Two-Pore-Domain Potassium Channel TREK–1 Mediates Pulmonary Fibrosis through Macrophage M2 Polarization and by Direct Promotion of Fibroblast Differentiation. BIOMEDICINES. 2023 May;11(5):1279  
[IF=3.212] Shang-Ping Zhao. et al. Long Non-coding RNA FENDRR Modulates Autophagy Through Epigenetic Suppression of ATG7 via Binding PRC2 in Acute Pancreatitis. 2021 Jan 08  
[IF=2.985] Tao Song. et al. FLOT2 upregulation promotes growth and invasion by interacting and stabilizing EphA2 in gliomas. Biochem Bioph Res Co. 2021 Apr;548:67  other ;  
[IF=2.967] An Liu. et al. MiRNA-3662 reverses the gemcitabine resistance in pancreatic cancer through regulating the tumor metabolism. 2021 May 15  Other ;  
[IF=2.737] A. Liu. et al. Circ_03955 promotes pancreatic cancer tumorigenesis and Warburg effect by targeting the miR-3662/HIF-1α axis. 2021 Apr 17  Other ;  
[IF=2.311] Yang H et al. Long non-coding RNA EWSAT1 contributes to the proliferation and invasion of glioma by sponging miR?152?3p. Oncol Lett. 2020 Aug;20(2):1846-1854.  WB ;  
[IF=2.089] Zhongxue Ye. et al. DNMT3B attenuated the inhibition of TET3 on epithelial-mesenchymal transition in TGF-β1-induced ovarian cancer by methylating the TET3 promoter. REPROD BIOL. 2022 Dec;22:100701  Other ;  Other.  
[IF=1.902] Xiong Qian. et al. Tectorigenin regulates migration, invasion, and apoptosis in dexamethasone‐induced human airway epithelial cells through up‐regulating miR‐222‐3p. 2021 Feb 04  other ;  
[IF=1.894] Qian D et al. Roles of CCNB2 and NKX3-1 in Nasopharyngeal Carcinoma. Cancer Biother Radiopharm. 2020 Apr;35(3):208-213.  WB ;  human.  
[IF=1.871] Bi H et al. Interleukin?8 promotes cell migration via CXCR1 and CXCR2 in liver cancer. ONCOLOGY LETTERS.2019 18: 4176-4184.  WB ;  
[IF=1.785] Hui Yang. et al. Upregulated LINC00319 aggravates neuronal injury induced by oxygen?glucose deprivation via modulating miR?200a?3p. Exp Ther Med. 2021 Aug;22(2):1-10  Other ;  
[IF=1.785] An J et al. A clinical and basic study of optimal endometrial preparation protocols for patients with infertility undergoing frozen?thawed embryo transfer. Exp Ther Med . 2020 Sep;20(3):2191-2199.  WB ;  
[IF=1.777] Wenbo Zhao. et al. The effect of the WKYMVm peptide on promoting mBMSC secretion of exosomes to induce M2 macrophage polarization through the FPR2 pathway. J Orthop Surg Res. 2021 Dec;16(1):1-14  Other ;  
[IF=1.492] Xu S et al. Glycoprotein nonmetastatic melanoma protein B accelerates tumorigenesis of cervical cancer in vitro by regulating the Wnt/β-catenin pathway.(2018) Braz. J. Med. Biol. Res.;52(1)  WB ;  
保存條件 RIPA裂解液于2-8℃可保存一年;PMSF置于-20 ℃保存。.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹

簡介:<br />
RIPA裂解液(RIPA Lysis Buffer)是一種傳統(tǒng)的細(xì)胞組織快速裂解液。RIPA裂解液裂解得到的蛋白樣品可以用于常規(guī)的Western、IP等。<br />
RIPA裂解液的主要成分為主要成分為50mM Tris(pH 7.4),150mM NaCl,1% NP-40,0.5% sodium deoxycholate,0.1% SDS、EDTA等,另加有蛋白酶抑制劑Aprotinin等可以有效抑制蛋白降解。<br />
<br />
使用說明:<br />
根據(jù)使用量,取每1ml RIPA 加入10ul PMSF,使PMSF的最終濃度為1mM?;靹騻溆茫≒MSF現(xiàn)用現(xiàn)加)。<br />
1、樣品前處理:<br />
a)對于貼壁細(xì)胞:去除培養(yǎng)液,用 PBS、生理鹽水或無血清培養(yǎng)液洗一遍。按照 6 孔板每孔細(xì)胞量加入150-250 ul裂解液的比例加入裂解液。用槍吹打數(shù)下,使裂解液和細(xì)胞充分接觸。<br />
b)對于懸浮細(xì)胞:離心收集細(xì)胞,用手指把細(xì)胞用力彈散。按照6孔板每孔細(xì)胞量加入150-250ul裂解液的比例加入裂解液,再用手指輕彈以充分裂解細(xì)胞。充分裂解后應(yīng)沒有明顯的細(xì)胞沉淀。如果細(xì)胞量較多,必需分裝成50-100萬細(xì)胞/管,然后再裂解。<br />
c)對于組織樣品: 把組織剪切成細(xì)小的碎片。按照每20mg組織加入150-250 ul裂解液的比例加入裂解液。 (如果裂解不充分可以適當(dāng)添加更多的裂解液,如果需要高濃度的蛋白樣品,可以適當(dāng)減少裂解液的用量)。<br />
用玻璃勻漿器勻漿,直至充分裂解。<br />
2、后處理:<br />
將裂解后的樣品10000-14000g離心3-5分鐘,取上清,即可進(jìn)行后續(xù)的PAGE、Western和免疫沉淀等操作。<br />
<br />
注意事項(xiàng):<br />
1、 因SDS在低溫易析出,如發(fā)現(xiàn)RIPA有沉淀,請先使其恢復(fù)室溫,沉淀即可溶解。<br />
2、 裂解蛋白的所有步驟都需在冰上或4℃進(jìn)行。<br />

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