| 產(chǎn)品編號(hào) | BA00102 |
| 英文名稱 | Annexin V-AF488 Apoptosis Detection Kit |
| 中文名稱 | Annexin V-AF488細(xì)胞凋亡檢測(cè)試劑盒 |
| 別 名 | Annexin V-AF488/PI Apoptosis Detection Kit; Annexin V-AF488 Apoptosis Detection Kit; Annexin V-AF488/PI Kit; Annexin V-AbBy Fluor? 488/PI Kit; Annexin V-AF488/PI雙染細(xì)胞凋亡檢測(cè)試劑盒; Annexin V-AF488-PI細(xì)胞凋亡檢測(cè)試劑盒; AF488 Annexin V/PI細(xì)胞凋亡檢測(cè)試劑盒; Annexin V-AF488/PI雙染細(xì)胞凋亡檢測(cè)試劑盒; ANNEXIN V-AF488/PI凋亡檢測(cè)試劑盒; 細(xì)胞凋亡檢測(cè)試劑盒; 細(xì)胞凋亡試劑盒; |
| 保存條件 | Store at 4℃. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
磷脂酰絲氨酸（PS）是一種帶負(fù)電荷的磷脂，正常細(xì)胞中，PS只分布在細(xì)胞膜脂質(zhì)雙層的內(nèi)側(cè)，而在細(xì)胞凋亡早期，細(xì)胞膜 PS由脂膜內(nèi)側(cè)翻向細(xì)胞膜外側(cè)，使PS暴露在細(xì)胞膜外表面。Annexin V是一種分子量為35~36kD 的Ca2+依賴性磷脂結(jié)合蛋白，與磷脂酰絲氨酸有高度親和力，故可通過細(xì)胞外側(cè)暴露的磷脂酰絲氨酸與凋亡早期細(xì)胞的胞膜結(jié)合。因此Annexin V被公認(rèn)為檢測(cè)細(xì)胞早期凋亡的靈敏指標(biāo)之一。 將Annexin V進(jìn)行Alexa Fluor488標(biāo)記，以標(biāo)記了的Annexin V作為探針，利用熒光顯微鏡或流式細(xì)胞儀可檢測(cè)細(xì)胞凋亡的發(fā)生。碘化丙啶（Propidium Iodide, PI）是一種核酸染料，它不能透過正常細(xì)胞或早期凋亡細(xì)胞的完整的細(xì)胞膜，但對(duì)凋亡中晚期的細(xì)胞和壞死細(xì)胞，PI能夠透過細(xì)胞膜而使細(xì)胞核染紅。因此采用Annexin V與PI雙染的方法，就可以將處于不同凋亡時(shí)期的細(xì)胞區(qū)分開來。 |
| 產(chǎn)品圖片 |
Jurkat cells (T-cell leukemia,human) treated with 10 μM of camptothecin for 4 hours(panel right) or untreated control(panel left). 1. Wash cells twice with cold PBS and then resuspend cells in 1×Binding Buffer at a concentration of 1×106 cells/ml. 2. Transfer 100 μl of the solution (1×105cells) to a 5 ml culture tube. 3. Add 5 μl of Annexin V-AF488. 4. Add 5 μl PI. 5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 6. Add 400 μl of 1×Binding Buffer to each tube.Analyze by flow cytometry within 1 hr.
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