| 產品編號 | BA00103 |
| 英文名稱 | Annexin V-AF647 Apoptosis Detection Kit |
| 中文名稱 | Annexin V-AF647細胞凋亡檢測試劑盒 |
| 別 名 | Annexin V-AF647/PI Apoptosis Detection Kit; Annexin V-AF647 Apoptosis Detection Kit; Annexin V-AbBy Fluor? 647/PI Kit; Annexin V-AbBy Fluor? 647/PI Kit; Annexin V-AF647/PI Kit; Annexin V-AF647/PI雙染細胞凋亡檢測試劑盒; Annexin V-AF647細胞凋亡檢測試劑盒; AF647 Annexin V/PI細胞凋亡檢測試劑盒; Annexin V-AF647/PI雙染細胞凋亡檢測試劑盒; ANNEXIN V- AF647凋亡檢測試劑盒; Annexin V-AF647/PI細胞凋亡檢測試劑盒; 細胞凋亡檢測試劑盒; 細胞凋亡試劑盒; |
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Specific References (2) | BA00103 has been referenced in 2 publications.
[IF=10.164] Wang D et al. Loss of 4.1N in epithelial ovarian cancer results in EMT and matrix-detached cell death resistance.. Protein Cell. 2020 May 25. FCM ; Human.
[IF=5.714] Xiangming Liu. et al. Taxifolin ameliorates cigarette smoke-induced chronic obstructive pulmonary disease via inhibiting inflammation and apoptosis. INT IMMUNOPHARMACOL. 2023 Feb;115:109577
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| 保存條件 | Store at 4℃. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
磷脂酰絲氨酸（PS）是一種帶負電荷的磷脂，正常細胞中，PS只分布在細胞膜脂質雙層的內側，而在細胞凋亡早期，細胞膜 PS由脂膜內側翻向細胞膜外側，使PS暴露在細胞膜外表面。Annexin V是一種分子量為35~36kD 的Ca2+依賴性磷脂結合蛋白，與磷脂酰絲氨酸有高度親和力，故可通過細胞外側暴露的磷脂酰絲氨酸與凋亡早期細胞的胞膜結合。因此Annexin V被公認為檢測細胞早期凋亡的靈敏指標之一。 將Annexin V進行Alexa Fluor 647標記，以標記了的Annexin V作為探針，利用熒光顯微鏡或流式細胞儀可檢測細胞凋亡的發(fā)生。碘化丙啶（Propidium Iodide, PI）是一種核酸染料，它不能透過正常細胞或早期凋亡細胞的完整的細胞膜，但對凋亡中晚期的細胞和壞死細胞，PI能夠透過細胞膜而使細胞核染紅。因此采用Annexin V與PI雙染的方法，就可以將處于不同凋亡時期的細胞區(qū)分開來。 |
| 產品圖片 |
Jurkat cells (T-cell leukemia,human) treated with 10 μM of camptothecin for 4 hours(panel right) or untreated control(panel left).
1. Wash cells twice with cold PBS and then resuspend cells in 1×Binding Buffer at a concentration of 1×106 cells/ml.
2. Transfer 100 μl of the solution (1×105cells) to a 5 ml culture tube.
3. Add 5 μl of Annexin V-AF647.
4. Add 5 μl PI.
5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
6. Add 400 μl of 1×Binding Buffer to each tube.Analyze by flow cytometry within 1 hr.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
