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DAPI solution (Nuclear Labeling) (C02-04002)  
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10ml/120.00元
50ml/320.00元
大包裝/詢價(jià)

產(chǎn)品編號(hào) C02-04002
英文名稱 DAPI solution (Nuclear Labeling)
中文名稱 DAPI染色液(即用型)
別    名 Nuclear Labeling Kit; DAPI solution(ready-to-use); DAPI Staining Solution;   DAPI溶液(即用型) 10ug/ml; DAPI染液(即用型); DAPI溶液(即用型); 核染色試劑盒; 核染色試劑盒(即用型); 核染色試劑盒(即用型); DAPI(4’,6-二脒基-2-苯基吲哚二鹽酸鹽); 4’,6-二脒基-2-苯基吲哚二鹽酸鹽;
Specific References  (57)     |     C02-04002 has been referenced in 57 publications.
[IF=16.744] Lubin Zhou. et al. A self-pumping dressing with in situ modification of non-woven fabric for promoting diabetic wound healing. CHEM ENG J. 2022 Dec;:141108  
[IF=9.995] Zhifang An. et al. A homotetrameric hemoglobin expressed in alveolar epithelial cells increases blood oxygenation in high-altitude plateau pika (Ochotona curzoniae). CELL REP. 2022 Oct;41:111446  Other ;  Other.  
[IF=9.685] Zhang, Yuan. et al. Downregulating PDPK1 and taking phillyrin as PDPK1-targeting drug protect hepatocytes from alcoholic steatohepatitis by promoting autophagy. CELL DEATH DIS. 2022 Nov;13(11):1-14  
[IF=9.587] Shi Yunhong. et al. N,N-Dimethyl-3β-hydroxycholenamide attenuates neuronal death and retinal inflammation in retinal ischemia/reperfusion injury by inhibiting Ninjurin 1. J NEUROINFLAMM. 2023 Dec;20(1):1-18  
[IF=9.423] Yu Pang. et al. Bladder epithelial cell phosphate transporter inhibition protects mice against uropathogenic Escherichia coli infection. CELL REP. 2022 Apr;39:110698  Other ;  Other.  
[IF=9.304] Jiantuan Xiong. et al. miR-195-3p alleviates homocysteine-mediated atherosclerosis by targeting IL-31 through its epigenetics modifications. 2021 Sep 30  IF ;  Mouse.  
[IF=7.79] Zhaoyan Yang. et al. Water-dispersed CsPbBr3 Nanocrystals for Single Molecule Localization Microscopy with High Location Accuracy for Targeted Bioimaging. Nanoscale. 2022 Mar;:  Other ;  Other.  
[IF=7.675] Jiahui Tang. et al. Increased Mobile Zinc Regulates Retinal Ganglion Cell Survival via Activating Mitochondrial OMA1 and Integrated Stress Response. ANTIOXIDANTS-BASEL. 2022 Oct;11(10):2001  Other ;  Other.  
[IF=7.376] Li-Ming Yu. et al. Activation of PKG-CREB-KLF15 by melatonin attenuates Angiotensin II-induced vulnerability to atrial fibrillation via enhancing branched-chain amino acids catabolism. Free Radical Bio Med. 2021 Dec;:  IF ;  Mouse.  
[IF=7.367] Yitian Du. et al. Engineered Microglia Potentiate the Action of Drugs against Glioma Through Extracellular Vesicles and Tunneling Nanotubes. 2021 Feb 28  IF,IHC ;  
[IF=7.243] Junping Liu. et al. JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1. GENES DIS. 2022 Aug;:  Other ;  Other.  
[IF=6.633] Li-Ming Yu. et al. Icariin attenuates excessive alcohol consumption-induced susceptibility to atrial fibrillation through SIRT3 signaling. BBA-MOL BASIS DIS. 2022 Oct;1868:166483  
[IF=6.63] Guo, Zhi-chen. et al. Porphyromonas gingivalis promotes the progression of oral squamous cell carcinoma by activating the neutrophil chemotaxis in the tumour microenvironment. CANCER IMMUNOL IMMUN. 2022 Dec;:1-17  
[IF=6.543] Yu Xing. et al. Changes of Functional, Morphological, and Inflammatory Reactions in Spontaneous Peripheral Nerve Reinnervation after Thermal Injury. Oxid Med Cell Longev. 2022;2022:9927602  Other ;  Other.  
[IF=5.895] Xinling Wang. et al. Enhancement of BCAT2-Mediated Valine Catabolism Stimulates β-Casein Synthesis via the AMPK-mTOR Signaling Axis in Bovine Mammary Epithelial Cells. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXX  Other ;  Other.  
[IF=5.895] Sheng-Zhong Han. et al. miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation. J AGR FOOD CHEM. 2022;70(33):10121–10133  Other ;  Other.  
[IF=5.561] Yu, Li-Ming. et al. Inhibition of ferroptosis by icariin treatment attenuates excessive ethanol consumption-induced atrial remodeling and susceptibility to atrial fibrillation, role of SIRT1. APOPTOSIS. 2023 Jan;:1-20  
[IF=5.555] Pengsen Wu. et al. IL-11 Is Elevated and Drives the Profibrotic Phenotype Transition of Orbital Fibroblasts in Thyroid-Associated Ophthalmopathy. Front Endocrinol. 2022; 13: 846106  Other ;  Other.  
[IF=5.552] Zhou L et al. Levo-corydalmine Attenuates Vincristine-Induced Neuropathic Pain in Mice by Upregulating the Nrf2/HO-1/CO Pathway to Inhibit Connexin 43 Expression. Neurotherapeutics. 2019 Oct 15.  IHF ;  
[IF=5.279] Min-Min Hu. et al. Sesamol Reverses Myofiber-Type Conversion in Obese States via Activating the SIRT1/AMPK Signal Pathway. J Agr Food Chem. 2022;70(7):2253–2264  Other ;  Other.  
[IF=5.085] Shuang-Yan Chang. et al. miR-320 regulates myogenesis by targeting growth factor receptor-bound protein-2 and ameliorates myotubes atrophy. INT J BIOCHEM CELL B. 2022 Jun;147:106212  Other ;  Other.  
[IF=5.08] Ye, Fan. et al. Rutin ameliorates inflammatory pain by inhibiting P2X7 receptor in mast cells. J PHYSIOL BIOCHEM. 2022 Dec;:1-9  
[IF=4.831] Lu Qi. et al. A newly identified small molecular compound acts as a protein kinase inhibitor to suppress metastasis of colorectal cancer. Bioorg Chem. 2021 Feb;107:104625  
[IF=4.803] Xing Du. et al. The mevalonate pathway promotes the metastasis of osteosarcoma by regulating YAP1 activity via RhoA. Genes Dis. 2020 Nov;:  IF ;  
[IF=4.627] Xiong X et al. Extracellular matrix derived from human urine-derived stem cells enhances the expansion, adhesion, spreading, and differentiation of human periodontal ligament stem cells. Stem Cell Res Ther. 2019 Dec 18;10(1):396.  Other ;  
[IF=4.599] Jimei Li. et al. A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors (Myospalax Baileyi). Front Genet. 2022; 13: 824049  Other ;  Other.  
[IF=4.529] Zha Zheng. et al. Study on the Anti-demyelination Mechanism of Bu-Shen-Yi-Sui Capsule in the Central Nervous System Based on Network Pharmacology and Experimental Verification. MEDIAT INFLAMM. 2022;2022:9241261  
[IF=4.473] Xudong Guo. et al. ARID3A plays a key regulatory role in palmitic acid-stimulated milk fat synthesis in mouse mammary epithelial cells. CELL BIOL INT. 2023 Feb;:  
[IF=4.268] Lei Yu. et al. Oxocrebanine: A Novel Dual Topoisomerase inhibitor, Suppressed the Proliferation of Breast Cancer Cells MCF-7 by Inducing DNA Damage and Mitotic Arrest. Phytomedicine. 2021 Apr;84:153504  other ;  
[IF=4.249] Zhou?L et al. Levo-corydalmine alleviates vincristine-induced neuropathic pain in mice by inhibiting an NF-kappa B-dependent CXCL1/CXCR6 signaling pathway. Neuropharmacology. 2018 Jun;135:34-47.  IF ;  
保存條件 Store at 2-8℃ for one year.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 基本信息:
λ Ex/λ Em(結(jié)合 DNA)= 360/460 nm
CAS 號(hào):28718-90-3
分子量:350.25
分子式:C16H17Cl2N5

產(chǎn)品簡(jiǎn)介:
DAPI即4',6-二脒基-2-苯基吲哚,能與DNA強(qiáng)力結(jié)合的熒光染料,常用與熒光顯微鏡及共聚焦觀測(cè)。DAPI可以透過(guò)完整的細(xì)胞膜,用于活細(xì)胞和固定細(xì)胞的染色。分子式為C16H15N5?2HCl ,分子量為350.25。
DAPI可以穿透細(xì)胞膜與細(xì)胞核中的雙鏈DNA結(jié)合而發(fā)揮標(biāo)記的作用,可以產(chǎn)生比DAPI自身強(qiáng)20多倍的熒光。顯微鏡下可以看到顯藍(lán)色熒光的細(xì)胞,熒光顯微鏡觀察細(xì)胞標(biāo)記的效率高(幾乎為100 %) ,且對(duì)活細(xì)胞無(wú)毒副作用。DAPI染色常用于細(xì)胞凋亡檢測(cè),染色后用熒光顯微鏡觀察或流式細(xì)胞儀檢測(cè)。DAPI也常用于普通的細(xì)胞核染色以及某些特定情況下的雙鏈DNA染色。細(xì)胞經(jīng)熱激處理后用DAPI染色3分鐘,在熒光顯微鏡下可以看到到細(xì)胞核的形態(tài)變化。
DAPI的最大激發(fā)波長(zhǎng)為340nm,最大發(fā)射波長(zhǎng)為488nm, DAPI和雙鏈DNA結(jié)合后,最大激發(fā)波長(zhǎng)為360nm,最大發(fā)射波長(zhǎng)為460nm。
本DAPI染色液(DAPI Staining Solution)可以直接用于固定細(xì)胞或組織的細(xì)胞核染色。

使用方法:
1. 對(duì)于固定后的細(xì)胞或組織樣品,適當(dāng)洗滌去除固定劑。如果需要進(jìn)行免疫熒光染色,可先進(jìn)行免疫熒光染色,染完畢后再進(jìn)行DAPI染色。如果不需要進(jìn)行其它染色,則直接進(jìn)行DAPI染色。
2. 對(duì)于貼壁細(xì)胞或組織切片,加入少量DAPI染色液,覆蓋住樣品即可。對(duì)于懸浮細(xì)胞,至少加入待染色樣品體積3倍的色液,混勻。
3. 室溫孵育5-10分鐘。
4. 吸除DAPI染色液,用TBST、PBS或生理鹽水洗滌2-3次,每次3-5分鐘,洗掉未結(jié)合的DAPI。
5. 用帶有360nm激發(fā)波長(zhǎng),460nm發(fā)射波長(zhǎng)的濾光片的熒光顯微鏡觀察細(xì)胞。

注意事項(xiàng):
1. 熒光染料都存在淬滅的問(wèn)題,建議染色后盡量當(dāng)天完成檢測(cè)。
2. 為減緩熒光淬滅可以使用抗熒光淬滅封片液(產(chǎn)品編號(hào):C02-04003)。
3. DAPI對(duì)人體有一定刺激性,請(qǐng)注意適當(dāng)防護(hù),為了您的安全和健康,使用時(shí)戴一次性手套操作。

產(chǎn)品圖片
小鼠肺 石蠟切片 細(xì)胞核呈藍(lán)色
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