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Rabbit Anti-SV2A  antibody (bs-21382R)  
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產(chǎn)品編號 bs-21382R
英文名稱 Rabbit Anti-SV2A  antibody
中文名稱 突觸泡蛋白2A抗體
別    名 Synaptic vesicle protein 2A; Synaptic vesicle glycoprotein 2A; Synaptic vesicle protein 2A; Synaptic vesicle protein 2; Sv2; Kiaa0736; SV2A_HUMAN.  
研究領(lǐng)域 細(xì)胞生物  細(xì)胞膜受體  糖蛋白  線粒體  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse,Rat (predicted: Human,Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 83kDa
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SV2A: 621-720/742 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 SV2s (Synaptic Vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. SV2A is expressed ubiquitously throughout the brain. SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2A is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium-stimulated exocytosis. SV2A is a highly glycosylated synaptic vesicle protein with homology to transmembrane transporters.

Function:
Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles (By similarity).

Subunit:
Interacts with SYT1/synaptotagmin-1 in a calcium-dependent manner. Binds the adapter protein complex AP-2.

Subcellular Location:
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.

Post-translational modifications:
Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1.

Similarity:
Belongs to the major facilitator superfamily.

SWISS:
Q7L0J3

Gene ID:
9900

Database links:
Entrez Gene: 9900 Human

Entrez Gene: 64051 Mouse

Entrez Gene: 117559 Rat

Omim: 185860 Human

SwissProt: Q29397 Cow

SwissProt: Q7L0J3 Human

SwissProt: Q9JIS5 Mouse

SwissProt: Q02563 Rat

Unigene: 516153 Human

Unigene: 200365 Mouse

Unigene: 11264 Rat



突觸泡蛋白2A(SV2A)是一類跨膜糖蛋白,定位于脊椎動物神經(jīng)元及內(nèi)分泌細(xì)胞,與神經(jīng)遞質(zhì)的釋放、內(nèi)分泌泡胞吐作用、突觸泡穩(wěn)態(tài)的維持作用。
產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SV2A) Polyclonal Antibody, Unconjugated (bs-21382R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SV2A) Polyclonal Antibody, Unconjugated (bs-21382R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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