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Rabbit Anti-CDCA7  antibody (bs-22227R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-22227R
英文名稱 Rabbit Anti-CDCA7  antibody
中文名稱 細胞分裂周期相關蛋白
別    名 CDCA7; CDCA 7; CDCA-7; Cell division cycle associated 7; JPO1; CDCA7_HUMAN.  
Specific References  (1)     |     bs-22227R has been referenced in 1 publications.
[IF=5.307] Jian Chen. et al. Novel isatin-based hybrids as potential anti-rheumatoid arthritis drug candidates: Synthesis and biological evaluation. BIOORG CHEM. 2022 Nov;128:106063  IHC ;  Mouse.  
研究領域 腫瘤  細胞生物  免疫學  信號轉(zhuǎn)導  細胞周期蛋白  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow)
產(chǎn)品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 43kDa
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CDCA7 : 201-300/371 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 CDCA7 is a nuclear protein without a known function, although its high homology with the transcription factor JPO2 or RAM2 suggests that it is also a transcription factor. CDCA7 is one of many target genes regulated by the c-Myc transcription factor. Overexpression of CDCA7 occurs in a significant proportion of cancers and it may play an important role in tumorigenesis. Normally it is periodically expressed in the cell cycle, peaking at the G1 to S phase transition.

Function:
Participates in MYC-mediated cell transformation; induces anchorage-independent growth and clonogenicity in lymphoblastoid cells. Insufficient to induce tumorigenicity when overexpressed but contributes to MYC-mediated tumorigenesis. May play a role as transcriptional regulator.

Subcellular Location:
Nuclear

Tissue Specificity:
Ubiquitous with higher level in thymus and small intestine. Overexpressed in a large number of tumors, in blood from patients with acute myelogenous leukemia (AML) and in chronic myelogenous leukemia (CML) blast crisis.

SWISS:
Q9BWT1

Gene ID:
83879

Database links:
Entrez Gene: 83879 Human

Entrez Gene: 66953 Mouse

Entrez Gene: 311742 Rat

Omim: 609937 Human

SwissProt: Q9BWT1 Human

SwissProt: Q9D0M2 Mouse

SwissProt: Q4KM91 Rat



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDCA7) Polyclonal Antibody, Unconjugated (bs-22227R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDCA7) Polyclonal Antibody, Unconjugated (bs-22227R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDCA7) Polyclonal Antibody, Unconjugated (bs-22227R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDCA7) Polyclonal Antibody, Unconjugated (bs-22227R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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