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Rabbit Anti-Cyclin B2  antibody (bsm-52045R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bsm-52045R
英文名稱 Rabbit Anti-Cyclin B2  antibody
中文名稱 周期素B2重組兔單抗
別    名 Cyclin-B2; CycB2; G2 mitotic specific cyclin B2; G2/mitotic specific cyclin B2; CCNB2_HUMAN.  
研究領域 細胞生物  免疫學  細胞周期蛋白  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 2F4
交叉反應 Human,Mouse,Rat
產(chǎn)品應用 WB=1:200-1000,IHC-P=1:50-200,IHC-F=1:50-200,ICC/IF=1:50-200,IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 45kDa
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human Cyclin B2 protein (1-150a) 
亞    型
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Cyclin B2 belongs to a the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin B2 has been shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins.

Function:
Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Subunit:
Interacts with the CDK1 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex.

Similarity:
Belongs to the cyclin family. Cyclin AB subfamily.

SWISS:
O95067

Gene ID:
9133

Database links:

Entrez Gene: 9133 Human

Entrez Gene: 12442 Mouse

Entrez Gene: 363088 Rat

Omim: 602755 Human

SwissProt: O95067 Human

SwissProt: P30276 Mouse

Unigene: 194698 Human

Unigene: 22592 Mouse



產(chǎn)品圖片
Western blot analysis of Cyclin B2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (bsm-52045R??, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cyclin B2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (bsm-52045R, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cyclin B2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( bsm-52045R, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC staining of Cyclin B2 in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (bsm-52045R, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor?488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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