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Mouse Anti-ALDH3A2  antibody (bsm-51470M)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bsm-51470M
英文名稱 Mouse Anti-ALDH3A2  antibody
中文名稱 ALDH3A2單克隆抗體
別    名 Ahd 3; Ahd 3r; Ahd3; Ahd3 r; AL3A2_HUMAN; Aldehyde dehydrogenase 10; Aldehyde dehydrogenase 3; Aldehyde dehydrogenase 3 family, member A2; Aldehyde dehydrogenase family 3 member A2; Aldehyde dehydrogenase family 3, subfamily A2; Aldehyde dehydrogenase, family 3, subfamily A, member 2; ALDH10; Aldh3; Aldh4; Aldh4 r; Aldh4r; FALDH; Fatty aldehyde dehydrogenase; FLJ20851; Microsomal aldehyde dehydrogenase; msALDH; SLS.  
研究領(lǐng)域 腫瘤  心血管  細(xì)胞生物  神經(jīng)生物學(xué)  信號轉(zhuǎn)導(dǎo)  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 AL03
交叉反應(yīng) Human,Mouse
產(chǎn)品應(yīng)用 WB=1:500-1000,IHC-P=1:50-100,IHC-F=1:400-800,IF=1:25
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 55kDa
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human ALDH3A2. 
亞    型 IgG1,IgK
純化方法 affinity purified by Protein G
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Aldehyde dehydrogenases (ALDHs) mediate the NADP+-dependent oxidation of aldehydes into acids and play an important role in the detoxification of alcohol-derived acetaldehyde, as well as in lipid peroxidation and in the metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH3A2 (aldehyde dehydrogenase 3 family, member A2), also known as SLS, FALDH or ALDH10, is a 485 amino acid single-pass membrane protein that localizes to the cytoplasmic side of the endoplasmic reticulum and belongs to the aldehyde dehydrogenase family. Expressed in a variety of tissues, including liver, heart, lung, brain, kidney and placenta, ALDH3A2 catalyzes the NAD+-dependent oxidation of long-chain aliphatic aldehydes to fatty acids, a process that is necessary for detoxification and lipid metabolism. Defects in the gene encoding ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS), an autosomal recessive neurocutaneous disorder characterized by severe mental retardation, seizures and speech defects. Multiple isoforms of ALDH3A2 exist due to alternative splicing events.

Function:
Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length.

Subcellular Location:
Endoplasmic reticulum membrane.

DISEASE:
Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS) [MIM:270200]. SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization). Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects.

Similarity:
Belongs to the aldehyde dehydrogenase family.

SWISS:
P51648

Gene ID:
224

Database links:

Entrez Gene: 224 Human

SwissProt: P51648 Human




產(chǎn)品圖片
Sample: Lane 1: Heart (Mouse) tissue lysates Primary: Anti-ALDH3A2 (bsm-51470M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 45 kD
Sample: Lane 1: WiDr cell lysates Primary: Anti-ALDH3A2 (bsm-51470M) at 1:1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ALDH3A2) Monoclonal Antibody, Unconjugated (bsm-51470M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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