| 產(chǎn)品編號 | bsm-51747M |
| 英文名稱 | Mouse Anti-SULT1A1 antibody |
| 中文名稱 | SULT1A1單克隆抗體 |
| 別 名 | ST1A1_HUMAN; SULT6B1; Aryl sulfotransferase 1; HAST1/HAST2; Human aryl sulfotransferase mRNA complete cds; P PST 1; P PST; P-PST 1; Phenol sulfating phenol sulfotransferase 1; Phenol sulfotransferase 1; Phenol-sulfating phenol sulfotransferase 1; PST; ST1A1; ST1A3; STP; STP1; Sulfotransferase 1A1; Sulfotransferase family 1A phenol preferring member 1; Sulfotransferase family cytosolic 1A phenol preferring member 1; Sulfotransferase phenol preferring 1; Thermostable phenol sulfotransferase; Ts PST; Ts-PST; TSPST1. |
| 研究領(lǐng)域 | 腫瘤 信號轉(zhuǎn)導(dǎo) 腫瘤細胞生物標志物 新陳代謝 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | C10G4 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human ST1A1. |
| 亞 型 | IgG1,Κ |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes one of two phenol sulfotransferases with thermostable enzyme activity. Multiple alternatively spliced variants that encode two isoforms have been identified for this gene. Function: Catalyzes the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Is also responsible for the sulfation and activation of minoxidil. Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk. Subunit: Homodimer. Subcellular Location: Cytoplasm. Tissue Specificity: Liver, lung, adrenal, brain, platelets and skin. Post-translational modifications: Substrate for botulinum ADP-ribosyltransferase. Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage. AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling. Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Similarity: Belongs to the sulfotransferase 1 family. SWISS: P50225 Gene ID: 6817 Database links: Entrez Gene: 6817 Human Entrez Gene: 20887 Mouse SwissProt: P50225 Human SwissProt: P52840 Mouse |
| 產(chǎn)品圖片 |
Sample:
Lane 1: HepG2 cell lysates
Primary: Anti-SULT1A1 (bsm-51747M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 30 kD
Sample:
Lane 1: HepG2 cell lysates
Lane 2: Human lung tissue lysates
Lane 3: Human liver tissue lysates
Lane 4: Mouse liver tissue lysates
Lane 5: Mouse kidney tissue lysates
Lane 6: Rat kidney tissue lysates
Primary: Anti-SULT1A1 (bsm-51747M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 30 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
