吃奶呻吟打开双腿做受动态图 -亚洲色偷偷色噜噜狠狠99网-日韩精品极品视频在线观看免费-来一水AV@lysav

掃碼關(guān)注公眾號(hào)           掃碼咨詢技術(shù)支持           掃碼咨詢技術(shù)服務(wù)
  
客服熱線:400-901-9800  客服QQ:4009019800  技術(shù)答疑  技術(shù)支持  質(zhì)量反饋  人才招聘  關(guān)于我們  聯(lián)系我們
亚洲欧美日韩在线不卡 ,欧美人妻精品一区二区三区
首頁(yè) > 產(chǎn)品中心 > 一抗 > 產(chǎn)品信息
Rabbit Anti-EDG1  antibody (bsm-52902R)  
~~~促銷代碼KT202411~~~
訂購(gòu)熱線:400-901-9800
訂購(gòu)郵箱:sales@xucheq.com
訂購(gòu)QQ:  400-901-9800
技術(shù)支持:techsupport@xucheq.com
說(shuō)明書: 50ul  100ul  
50ul/1400.00元
100ul/2500.00元
大包裝/詢價(jià)

產(chǎn)品編號(hào) bsm-52902R
英文名稱 Rabbit Anti-EDG1  antibody
中文名稱 內(nèi)皮細(xì)胞分化鞘脂G蛋白偶聯(lián)受體1重組兔單抗
別    名 CD363; CHEDG1; D1S3362; ECGF1; EDG1; EDG 1; Endothelial differentiation G-protein coupled receptor 1; Endothelial differentiation sphingolipid G protein coupled receptor 1; G protein coupled sphingolipid receptor; S1P receptor 1; S1P receptor Edg-1; S1P1; S1PR1; S1PR1_HUMAN; Sphingosine 1 phosphate receptor Edg 1; Sphingosine 1 phosphate receptor EDG1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; Sphingosine 1phosphate receptor type 1 S1P1.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  細(xì)胞凋亡  細(xì)胞粘附分子  G蛋白偶聯(lián)受體  內(nèi)皮細(xì)胞  細(xì)胞骨架  
抗體來(lái)源 Rabbit
克隆類型 Recombinant
交叉反應(yīng) Human,Mouse (predicted: Rat)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 44kDa
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human EDG1: 2-51/382 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Sphingosine-1-phosphate receptor 1 (S1P receptor 1 or S1P1), also known as endothelial differentiation gene 1 (EDG1) is a protein that in humans is encoded by the S1PR1 gene. S1PR1 is a G-protein-coupled receptor which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P). S1PR1 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR1-5). S1PR1 was originally identified as an abundant transcript in endothelial cells and it has an important role in regulating endothelial cell cytoskeletal structure, migration, capillary-like network formation and vascular maturation. In addition, S1PR1 signaling is important in the regulation of lymphocyte maturation, migration and trafficking.

Function:
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.

Post-translational modifications:
S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P21453

Gene ID:
1901

Database links:

Entrez Gene: 281135 Cow

Entrez Gene: 100050049 Horse

Entrez Gene: 1901 Human

Entrez Gene: 13609 Mouse

Entrez Gene: 29733 Rat

Omim: 601974 Human

SwissProt: Q5E9P3 Cow

SwissProt: P21453 Human

SwissProt: O08530 Mouse

SwissProt: P48303 Rat

Unigene: 154210 Human

Unigene: 982 Mouse

Unigene: 109455 Rat



研究發(fā)現(xiàn),S1P1與VEGF肩并肩相互協(xié)作,從而促進(jìn)血管生長(zhǎng)。VEGF是很多不同抗癌藥物的作用靶標(biāo)。血管新生在很多疾病是異常的。靶向S1P1和VEGF可能要比只使用VEGF抑制劑更加有效地治療疾??;S1P1是一種關(guān)鍵性的血管新生反應(yīng)性基因。研究人員證實(shí)當(dāng)新的血管網(wǎng)絡(luò)形成時(shí),由此產(chǎn)生的血液流動(dòng)激活血管內(nèi)皮細(xì)胞表面上的S1P1,并把信號(hào)傳遞到這些細(xì)胞內(nèi)部從而讓新形成的血管網(wǎng)絡(luò)穩(wěn)定化。因此阻斷S1P1可能有助于切斷給癌性腫瘤提供資源的血液供應(yīng)。
產(chǎn)品圖片
Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (bsm-52902R) at 1/500 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 μg/Lane. Predicted band size: 43 kDa Observed band size: 50 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (bsm-52902R) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (bsm-52902R, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-EDG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (bsm-52902R, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (bsm-52902R, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (bsm-52902R, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC staining of EDG1 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (bsm-52902R, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor?594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of EDG1 in HUVEC cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (bsm-52902R, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor?594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of EDG1 in SH-SY5Y cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (bsm-52902R, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor?594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
版權(quán)所有 2004-2026 www.xucheq.com 北京博奧森生物技術(shù)有限公司
通過(guò)國(guó)際質(zhì)量管理體系ISO 9001:2015 GB/T 19001-2016    證書編號(hào): 00124Q34771R2M/1100
通過(guò)國(guó)際醫(yī)療器械-質(zhì)量管理體系ISO 13485:2016 GB/T 42061-2022    證書編號(hào): CQC24QY10047R0M/1100
京ICP備05066980號(hào)-1         京公網(wǎng)安備110107000727號(hào)
性夜影院A片禁18免费看| 亚洲AV色香蕉一区二区三区| 孕妇滴着奶水做着爱A| 少妇伦子伦精品无码| 国产成A人亚洲精V品无码| 免费观看少妇全黄A片| 国产美女自慰在线观看| 无码AⅤ精品一区二区三区浪潮| 亲爱的老师4中字韩国电影| 帅小伙自慰VIDEOGAY男男| 999国产精品999久久久久久| 玩弄JAPAN白嫩少妇HD小说| 激情综合婷婷色五月蜜桃| 国产精品国色综合久久| 免费A级毛片在线播放不收费| 午夜福利电影 | 被喂春药蹂躏的欲仙欲死视频| 中文人妻AV久久人妻水蜜桃| 欧美老熟妇乱大交XXXXX | 香蕉人妻AV久久久久天天| 6080YYY午夜理论AA片| 精品久久香蕉国产线看观看亚洲| 日韩毛片| 精品久久久久久无码中文字幕一区| 国产精品黄在线观看免费软件| 国产无遮挡吃胸膜奶免费看| 厨房玩弄丝袜人妻系列国产电影| 亚洲精品乱码久久久久久不卡| 巨胸爆乳美女露双奶头挤奶| 国产亚洲精品久久久久久| 教子做爰XXXX| 东京热在线| 欧美肥妇BWBWBWBXX| 无码人妻精品一区二区三区在线 | 国产AV亚洲精品久久久久| 自拍日韩亚洲一区在线| 凹凸久久人人澡超碰凹凸 | 国产又粗又猛又爽又黄| 野花香视频在线观看免费高清版| 亚洲欧美另类激情综合区蜜芽| 一个刚拔出来另一个|