| 產(chǎn)品編號(hào) | bsm-36203M |
| 英文名稱 | Mouse Anti-CD19 antibody |
| 中文名稱 | CD19單克隆抗體 |
| 別 名 | CD19_HUMAN; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen: CD19; B-lymphocyte surface antigen B4; Leu 12; Leu12; |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Monoclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | IHC-P=1:200-800,IHC-F=1:200-800,IF=1:200-800
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 59kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human CD19 protein |
| 亞 型 | IgG2b |
| 純化方法 | affinity purified by Protein A |
| 保存條件 | Store at 2–8 °C. Do not freeze. Return to 2–8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020] Function: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Subunit: Forms a complex with CD21, CD81 and CD225 in the membrane of mature B-cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409. Interacts with LYN. Subcellular Location: Membrane; Single-pass type I membrane protein. Post-translational modifications: Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. DISEASE: Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Similarity: Belongs to the selectin/LECAM family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P15391 Gene ID: 930 Database links: Entrez Gene: 930 Human Entrez Gene: 12478 Mouse Omim: 107265 Human SwissProt: P15391 Human SwissProt: P25918 Mouse Unigene: 652262 Human Unigene: 4360 Mouse CD19是一種質(zhì)膜蛋白質(zhì)，參與信號(hào)傳導(dǎo)作用。表達(dá)與前B細(xì)胞和成熟的B細(xì)胞膜表面，與B細(xì)胞的活化調(diào)節(jié)和發(fā)育調(diào)節(jié)相關(guān)，在T細(xì)胞和正常粒細(xì)胞上無(wú)表達(dá)。此抗體可以特異性識(shí)別CD19，主要用于標(biāo)記正常B細(xì)胞及腫瘤性B細(xì)胞。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD19 Monoclonal Antibody, Unconjugated(bsm-36203M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD19 Monoclonal Antibody, Unconjugated(bsm-36203M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD19 Monoclonal Antibody, Unconjugated(bsm-36203M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
|
