產(chǎn)品編號 | bsm-51336M |
英文名稱 | Mouse Anti-RPA2 antibody |
中文名稱 | 復制因子A蛋白2單克隆抗體 |
別 名 | RFA2_HUMAN; Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2 (RF-A protein 2); Replication protein A 34 kDa subunit (RP-A p34); REPA2; RPA32; RPA34; replication protein A2; RP-A p34; |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
交叉反應 | (predicted: Human,Mouse,Rat) |
產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 29kDa |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | Recombinant human RPA2 Protein |
亞 型 | IgG1,Κ |
純化方法 | affinity purified by Protein A |
緩 沖 液 | PBS with 0.02% Proclin300. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
產(chǎn)品介紹 |
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |