| 產(chǎn)品編號(hào) | bsm-41814M |
| 英文名稱(chēng) | Mouse Anti-TFRC antibody |
| 中文名稱(chēng) | 轉(zhuǎn)鐵蛋白受體（CD71）單克隆抗體 |
| 別 名 | ansferrin receptor protein 1; TFRC; CD 71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TR; sTfR; Transferrin receptor (p90 CD71); Transferrin Receptor Protein 1; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; TRFR; TFR1_HUMAN. |
| 抗體來(lái)源 | Mouse |
| 克隆類(lèi)型 | Monoclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=2ug/Test,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 檢測(cè)分子量 | 95 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human CD71 protein.: 89-760/760 |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015] 轉(zhuǎn)鐵蛋白及轉(zhuǎn)鐵蛋白受體(TfnrR)是機(jī)體鐵代謝中的重要因子，鐵是細(xì)胞生長(zhǎng)增殖、代謝的必需元素,轉(zhuǎn)鐵蛋白受體(TfR)介導(dǎo)的胞吞作用是細(xì)胞攝取鐵的主要途徑。腫瘤細(xì)胞因增殖生長(zhǎng)旺盛,對(duì)鐵的需求高,所以腫瘤細(xì)胞有比正常細(xì)胞更多的轉(zhuǎn)鐵蛋白受體的表達(dá)。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HT-1080 cell lysates
Primary: Anti-TFRC (bsm-41814M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 74/85 kDa
Observed band size: 95 kDa
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TFRC) monoclonal Antibody, Unconjugated (bsm-43165M) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:Hela.
Primary Antibody (green line): Mouse Anti-TFRC antibody (bsm-43165M)
Dilution: 2ug/Test;
Secondary Antibody (white blue line) : Goat anti-mouse IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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