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Rabbit Anti-ID1  antibody (bsm-54101R)  
~~~促銷代碼KT202411~~~
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說明書: 50ul  100ul  
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100ul/2500.00元
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產(chǎn)品編號(hào) bsm-54101R
英文名稱 Rabbit Anti-ID1  antibody
中文名稱 DNA結(jié)合抑制因子1重組兔單抗
別    名 bHLHb24; Class B basic helix-loop-helix protein 24; DNA binding protein inhibitor ID 1; DNA binding protein inhibitor ID1; DNA-binding protein inhibitor ID-1; Dominant negative helix loop helix protein; ID 1; ID; ID1; ID1_HUMAN; Inhibitor of Differentiation 1; Inhibitor of DNA binding 1.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  染色質(zhì)和核信號(hào)  信號(hào)轉(zhuǎn)導(dǎo)  轉(zhuǎn)錄調(diào)節(jié)因子  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號(hào) 5F1
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-1000,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 16kDa
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human ID1 protein 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 ID proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID proteins contribute to cell growth, senescence, differentiation and angiogenesis. Id1 mRNA is highly expressed in heart, lung and kidney and has lower expression in brain and liver. Two transcript variants encoding different isoforms have been found for this gene.

Function:
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.

Subunit:
Heterodimer with other HLH proteins. Interacts with COPS5.

Subcellular Location:
Cytoplasm. Nucleus.

Similarity:
Contains 1 bHLH (basic helix-loop-helix) domain.

SWISS:
P41134

Gene ID:
3397

Database links:

Entrez Gene: 3397 Human

Entrez Gene: 15901 Mouse

Entrez Gene: 25261 Rat

Omim: 600349 Human

SwissProt: P41134 Human

SwissProt: P20067 Mouse

SwissProt: P41135 Rat

Unigene: 504609 Human

Unigene: 444 Mouse

Unigene: 2113 Rat



產(chǎn)品圖片
Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug Lane 2: MCF-7 (Human) Cell Lysate at 30 ug Lane 3: U-2os (Human) Cell Lysate at 30 ug Lane 4: A549 (Human) Cell Lysate at 30 ug Lane 5: Hela (Human) Cell Lysate at 30 ug Primary: Anti-ID1 (bsm-54101R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 16 kD Observed band size: 25 kD
U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ID1) monoclonal Antibody, Unconjugated (bsm-54101R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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