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Mouse Anti-BrdU/PE-Cy5 Conjugated antibody (bsm-0917M-PE-Cy5)
訂購熱線:400-901-9800
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產(chǎn)品編號 bsm-0917M-PE-Cy5
英文名稱1 Mouse Anti-BrdU/PE-Cy5 Conjugated antibody
中文名稱 PE-Cy5標(biāo)記的5-溴脫氧尿嘧啶核苷/增殖標(biāo)志物單克隆抗體
別    名 Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
產(chǎn)品類型 小分子抗體 
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  發(fā)育生物學(xué)  染色質(zhì)和核信號  神經(jīng)生物學(xué)  藥物及化合物  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 1A7
交叉反應(yīng) Rat, 
產(chǎn)品應(yīng)用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 0.3071kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 bromodeoxyuridine conjugated to KLH
亞    型 IgG
純化方法 affinity purified by Protein G
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Proliferation Marker
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood (Campana et al., 1988), tissues (Schutte et al., 1987; Hayashi, et al., 1988), tumors (Hoshino et al., 1986; Morstyn et al., 1983), as well as for determining plasma cell labeling indices (Greipp et al., 1985).

Subcellular Location:
Nuclear.

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

增殖標(biāo)志物
BrdU(溴化脫氧尿嘧啶核苷)可以在體內(nèi)和體外摻入到處于S期的細(xì)胞所合成的DNA鏈中。此抗體可以與摻入到任何種屬細(xì)胞中的BrdU反應(yīng),與碘脫氧尿嘧啶有交叉反應(yīng),標(biāo)記摻有BrdU的S期細(xì)胞,主要用于研究各種不同的因素對正常/腫瘤組織的細(xì)胞增殖及動力學(xué)的研究。
用FITC標(biāo)記的抗BrdU IgG可用于流式細(xì)胞術(shù)定量檢測增值細(xì)胞。
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