產品編號 | bs-6152R-BF488 |
英文名稱1 | Rabbit Anti-GNAQ/BF488 Conjugated antibody |
中文名稱 | BF488標記的G蛋白α亞單位蛋白Q抗體 |
別 名 | G ALPHA q; G protein alpha Q; GAQ; Guanine nucleotide binding protein alpha q; Guanine nucleotide binding protein G q subunit alpha; GNAQ_HUMAN. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 腫瘤 心血管 細胞生物 神經生物學 信號轉導 G蛋白偶聯受體 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Rat, (predicted: Human, Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) |
產品應用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 42kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human GNAQ |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產品介紹 |
background: GNAQ belong to the family of heterotrimeric Guanine nucleotide-binding proteins proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. GNAQ is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta. in recent studies over expression of GNAQ is associated with cardiomyocyte apoptosis. Function: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity. Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity). Subunit: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Binds SLC9A3R1. Forms a complex with PECAM1 and BDKRB2. Interacts with PECAM1. Subcellular Location: Nucleus (By similarity). Membrane (By similarity). Nucleus membrane (By similarity). Note=Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes (By similarity). Tissue Specificity: Predominantly expressed in ovary, prostate, testis and colon. Similarity: Belongs to the G-alpha family. G(q) subfamily. Database links: UniProtKB/Swiss-Prot: P50148.4 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Gαq是G蛋白家族成員之一,是Gq蛋白的功能單位。其功能為傳導來自GPCR的信號到細胞內。研究證明,Gαq與免疫細胞活化、增殖、死亡、分化、遷移,細胞因子分泌以及自身免疫等均有非常密切的關系。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |