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Rabbit Anti-CD33/BF647 Conjugated antibody (bs-1514R-BF647)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-1514R-BF647
英文名稱1 Rabbit Anti-CD33/BF647 Conjugated antibody
中文名稱 BF647標(biāo)記的CD33抗體
別    名 CD33_HUMAN; CD 33; CD33 antigen; CD33 molecule; FLJ00391; gp67 ; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; p67 ; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; SIGLEC3; Siglec-3; myeloid cell surface antigen CD33 isoform 1 precursor.  
規(guī)格價(jià)格 100ul/2980元 購買        大包裝/詢價(jià)
說 明 書 100ul  
研究領(lǐng)域 免疫學(xué)  干細(xì)胞  細(xì)胞表面分子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse,  (predicted: Rat, )
產(chǎn)品應(yīng)用 Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 40/37kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse CD33
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.

Function:
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).

Subunit:
Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Monocytic/myeloid lineage cells.

Post-translational modifications:
Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11.
Phosphorylation of Tyr-358 is involved in binding to PTPN6.

Similarity:
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Database links:

Entrez Gene: 945 Human

Entrez Gene: 12489 Mouse

Entrez Gene: 690492 Rat

Omim: 159590 Human

SwissProt: P20138 Human

SwissProt: Q63994 Mouse

Unigene: 83731 Human

Unigene: 140157 Mouse




Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD33(Siglec-3)也是急性淋巴細(xì)胞白血病抗原,細(xì)胞表面跨膜糖蛋白.CD33表達(dá)于早期B淋巴細(xì)胞,某些粒性白細(xì)胞,骨髓基質(zhì)細(xì)胞,部分上皮組織及其起源的腫瘤細(xì)胞中也有表達(dá).主要應(yīng)用于某些惡性淋巴瘤和白血病的分型.
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