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Rabbit Anti-Transferrin receptor/CD71/Biotin Conjugated antibody (bs-1782R-Bio)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產品編號 bs-1782R-Bio
英文名稱1 Rabbit Anti-Transferrin receptor/CD71/Biotin Conjugated antibody
中文名稱 生物素標記的轉鐵蛋白受體抗體
別    名 CD 71; CD71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TFRC; TR; Transferrin receptor (p90 CD71); Transferrin Receptor Protein 1; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; Transferrin receptor protein 1, serum form; TRFR.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 腫瘤  心血管  細胞生物  免疫學  神經生物學  干細胞  糖尿病  細胞表面分子  細胞類型標志物  淋巴細胞  t-淋巴細胞  b-淋巴細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,  (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, )
產品應用 WB=1:50-200 ELISA=1:100-1000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 74/85kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD71
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.

Function:
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferring for an overlapping C-terminal binding site.

Subunit:
Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with Machupo arenavirus GPC.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Transferrin receptor protein 1, serum form: Secreted

Post-translational modifications:
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

Similarity:
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.

Database links:

Entrez Gene: 7037 Human

Entrez Gene: 22042 Mouse

Entrez Gene: 64678 Rat

Omim: 190010 Human

SwissProt: P02786 Human

SwissProt: Q62351 Mouse

SwissProt: Q99376 Rat

Unigene: 529618 Human

Unigene: 28683 Mouse

Unigene: 98672 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

轉鐵蛋白及轉鐵蛋白受體(TfnrR)是機體鐵代謝中的重要因子,鐵是細胞生長增殖、代謝的必需元素,轉鐵蛋白受體(TfR)介導的胞吞作用是細胞攝取鐵的主要途徑。腫瘤細胞因增殖生長旺盛,對鐵的需求高,所以腫瘤細胞有比正常細胞更多的轉鐵蛋白受體的表達。
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