| 產(chǎn)品編號 | bs-6661R-Bio |
| 英文名稱1 | Rabbit Anti-AQP0/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的水通道蛋白0抗體 |
| 別 名 | AQP 0; AQP0; Aquaporin; Aquaporin0; Lens fiber major intrinsic protein; LIM 1; LIM1; LIM-1; Major intrinsic protein 26kD; Major intrinsic protein of lens fiber; MIP 26; MIP; MIP26; MP 26; MP26; MIP_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 干細胞 通道蛋白 細胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 29kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AQP0 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein aquaporins. Aquaporin 0 or MIP26 (major intrinsic protein 26kD), and Aquaporin 1 has been the foundation of the growing family of aquaporins. The lens specific Aquaporin 0 represents up to 80% of total lens membrane protein. Defects in Aquaporin 0 are a cause of autosomal recessive congenital cataract. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Human Aquaporin 0 is a 263 amino acid transmembrane protein belonging to the MIP family. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C terminus are predicted to be cytoplasmic. Function: Water channel. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core. Subunit: Homotetramer. Homooctamer formed by head-to-head interaction between homotetramers from adjoining membranes. During early stages of lens development, interacts through its C-terminal region with Cx56 and GJA8/Cx45.6. Subcellular Location: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. Tissue Specificity: Major component of lens fiber gap junctions. Similarity: Contains 1 homeobox DNA-binding domain. Contains 2 LIM zinc-binding domains. Database links: Entrez Gene: 4284 Human Entrez Gene: 17339 Mouse Omim: 154050 Human SwissProt: P30301 Human SwissProt: P51180 Mouse Unigene: 574026 Human Unigene: 31625 Mouse Unigene: 396469 Mouse Unigene: 23532 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
