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Rabbit Anti-CD10/AP Conjugated antibody (bs-0527R-AP)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價

產(chǎn)品編號 bs-0527R-AP
英文名稱1 Rabbit Anti-CD10/AP Conjugated antibody
中文名稱 堿性磷酸酶(AP)標記的CD10抗體
別    名 Atriopeptidase; CALLA; CD 10; CD10; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24.11; Enkephalinase; EPN; gp100; Membrane metallo endopeptidase; Membrane metalloendopeptidase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10; Membrane metalloendopeptidase variant 1; Membrane metalloendopeptidase variant 2; MGC126681; MGC126707; MME; NEP; Neprilysin; Neutral endopeptidase 24.11; Neutral endopeptidase; NEP_HUMAN; Neprilysin; Skin fibroblast elastase; SFE.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領(lǐng)域 腫瘤  細胞生物  免疫學  干細胞  細胞表面分子  細胞類型標志物  淋巴細胞  b-淋巴細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,  (predicted: Mouse, Rat, )
產(chǎn)品應(yīng)用 WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 82kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD10
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing.

Function:
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein.

Post-translational modifications:
Myristoylation is a determinant of membrane targeting.
Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity.

Similarity:
Belongs to the peptidase M13 family.

Database links:

Entrez Gene: 4311 Human

Entrez Gene: 17380 Mouse

Entrez Gene: 24590 Rat

Omim: 120520 Human

SwissProt: P08473 Human

SwissProt: Q61391 Mouse

SwissProt: P07861 Rat

Unigene: 307734 Human

Unigene: 296022 Mouse

Unigene: 33598 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD10即共同型急性淋巴細胞白血病抗原,是II型跨膜糖蛋白.CD10表達于早期B淋巴細胞,某些粒性白細胞,骨髓基質(zhì)細胞,部分上皮組織及其起源的腫瘤細胞中也有表達.主要應(yīng)用于某些惡性淋巴瘤和白血病的分型.CD10是一種94kD的鋅依賴性細胞膜金屬結(jié)合蛋白,參與細胞神經(jīng)肽和激素肽的后分泌過程。它廣泛地分布于腎、肝、小腸、胎盤、脈絡(luò)膜叢、腦腺、腺皮質(zhì)和白細胞,CD10是生發(fā)中心細胞及所致淋巴瘤的標志物。
產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (Human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD10) Polyclonal Antibody, Unconjugated (bs-0527R) at 1:25 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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