| 產(chǎn)品編號 | bs-9663R-PE-Cy5 |
| 英文名稱1 | Rabbit Anti-E3 ubiquitin ligase/PE-Cy5 Conjugated antibody |
| 中文名稱 | PE-Cy5標記的E3泛素連接酶抗體 |
| 別 名 | C1orf166; E3 ubiquitin ligase; E3 ubiquitin protein ligase MUL1; FLJ12875; GIDE; Growth inhibition and death E3 ligase; MAPL; Mitochondrial anchored protein ligase; Mitochondrial ubiquitin ligase activator of NFKB 1; MUL1; MULAN; Putative NF kappa B activating protein 266; RING finger protein 218; RNF218; RP23-25C1.10-002. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞分化 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Pig, Rabbit, ) |
| 產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 40kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human E3 ubiquitin ligase/RNF218 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: E3 ubiquitin-protein ligase that plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Function: Exhibits weak E3 ubiquitin-protein ligase activity, but preferentially acts as a SUMO E3 ligase at physiological concentrations. Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Subunit: Homooligomer. Interacts with MAP3K7/TAK1. Interacts with UBC9. Interacts with and sumoylates DNM1L. Subcellular Location: Mitochondrion outer membrane; Multi-pass membrane protein. Peroxisome. Note: Transported in mitochondrion-derived vesicles from the mitochondrion to the peroxisome. Tissue Specificity: Widely expressed with highest levels in the heart, skeletal muscle, placenta, kidney and liver. Barely detectable in colon and thymus. Similarity: Contains 1 RING-type zinc finger. Database links: Entrez Gene: 79594 Human Entrez Gene: 68350 Mouse Omim: 612037 Human SwissProt: Q969V5 Human SwissProt: Q8VCM5 Mouse Unigene: 10101 Human Unigene: 103413 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
