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Rabbit Anti-Phospho-Cyclin B1 (Ser147)/AP Conjugated antibody (bs-3122R-AP)
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說(shuō) 明 書(shū): 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-3122R-AP
英文名稱1 Rabbit Anti-Phospho-Cyclin B1 (Ser147)/AP Conjugated antibody
中文名稱 堿性磷酸酶(AP)標(biāo)記的磷酸化周期素B1抗體
別    名 Cyclin B1 (phospho S147); Cyclin B1 (phospho-Ser147); p-Cyclin B1 (S147); p-Cyclin B1 (Ser147); p-Cyclin B1 (phospho S147); CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1.  
規(guī)格價(jià)格 100ul/2980元 購(gòu)買(mǎi)        大包裝/詢價(jià)
說(shuō) 明 書(shū) 100ul  
產(chǎn)品類(lèi)型 磷酸化抗體 
研究領(lǐng)域 腫瘤  細(xì)胞生物  細(xì)胞周期蛋白  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human,  (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, )
產(chǎn)品應(yīng)用 WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 48kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser147
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].

Function:
Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Subunit:
Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization.

Subcellular Location:
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome.

Post-translational modifications:
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site

Similarity:
Belongs to the cyclin family. Cyclin AB subfamily.

Database links:

Entrez Gene: 891 Human

Entrez Gene: 268697 Mouse

Entrez Gene: 25203 Rat

Omim: 123836 Human

SwissProt: P14635 Human

SwissProt: P24860 Mouse

SwissProt: P30277 Rat

Unigene: 23960 Human

Unigene: 260114 Mouse

Unigene: 380027 Mouse

Unigene: 482545 Mouse

Unigene: 9232 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

主要出現(xiàn)在G2期。Cyclin B是細(xì)胞周期調(diào)節(jié)必不可少的條件。 細(xì)胞周期素B1是細(xì)胞周期調(diào)節(jié)因子,它的異常表達(dá)將導(dǎo)致細(xì)胞周期發(fā)生紊亂,致使腫瘤形成.
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