| 產(chǎn)品編號(hào) | bs-3165R-Gold |
| 英文名稱1 | Rabbit Anti-Phospho-Gab1 (Tyr627)/Gold Conjugated antibody |
| 中文名稱 | 膠體金標(biāo)記的磷酸化接頭蛋白Gab 1抗體 |
| 別 名 | GAB1 (phospho Y627); Gab1(Phospho-Tyr627); p-Gab1 (Tyr627); GRB 2 associated binder 1; GRB 2 associated binding protein 1; GRB2 associated binding protein 1 isoform a; GRB2 associated binding protein 1 isoform bl; Gab 1; Gab1; GAB1_HUMAN; GRB2 associated binder 1; GRB2 associated binding protein 1 isoform b; GRB2-associated binder 1; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1. |
| 規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買(mǎi) 大包裝/詢價(jià) |
| 說(shuō) 明 書(shū) | 100ul（10nm 15nm 35nm） |
| 產(chǎn)品類(lèi)型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 心血管 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞膜受體 糖尿病 內(nèi)分泌病 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 76kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Gab1 around the phosphorylation site of Tyr627 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 產(chǎn)品介紹 |
background: Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus, activating mitogenic as well as other responses. Critical components of this process include adapter proteins such as Shc, IRS-1 and Gab 1 (GRB-associated binder-1) that lack detectable catalytic activity (1-3,8). Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c Met), and epidermal growth factor receptor (EGF), as well as in response to cell cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF. Function: Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Subunit: Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2A, GRB2, GAB1, PIK3R1 and SOS1. Post-translational modifications: Phosphorylated in response to FGFR1 activation. Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Similarity: Belongs to the GAB family. Contains 1 PH domain. Database links: Entrez Gene: 2549 Human Entrez Gene: 14388 Mouse Omim: 604439 Human SwissProt: Q13480 Human SwissProt: Q9QYY0 Mouse Unigene: 618456 Human Unigene: 80720 Human Unigene: 277409 Mouse Unigene: 1725 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Gab1作為一種分布廣泛的接頭蛋白，幾乎能連接所有類(lèi)型的受體（如酪氨酸激酶受體、G-蛋白偶聯(lián)受體、細(xì)胞因子與抗原受體等，Gab1蛋白屬于接頭蛋白Gab家族,該家族蛋白因能與生長(zhǎng)因子受體結(jié)合蛋白2（Grb2）相結(jié)合而得名。作為接頭蛋白,Gab1蛋白能被多種受體酪氨酸激酶或非受體酪氨酸激酶激活,接受胞外多種生長(zhǎng)因子、細(xì)胞因子和一些T/B細(xì)胞抗原受體的刺激,介導(dǎo)PI3K/Akt和Ras/MAPK等多條信號(hào)轉(zhuǎn)導(dǎo)途徑,具有促進(jìn)細(xì)胞生長(zhǎng)、遷移、調(diào)節(jié)免疫等多種生物學(xué)功能,與糖尿病、腫瘤、心血管疾病等的發(fā)生發(fā)展密切相關(guān)。 |
