| 產(chǎn)品編號 | bs-2774R-BF647 |
| 英文名稱1 | Rabbit Anti-prox1/BF647 Conjugated antibody |
| 中文名稱 | BF647標記的prox-1抗體 |
| 別 名 | Homeobox prospero like protein; Homeobox prospero like protein PROX1; Prospero related homeobox 1;prox-1; PROX 1. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 心血管 免疫學 神經(jīng)生物學 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產(chǎn)品應用 | Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 81kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human prox-1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Prox1 is a prospero-related homeobox gene. Its expression pattern suggests it has a role in a variety of embryonic tissues and may play a fundamental role in early development of the CNS. It may regulate gene expression and the development of postmitotic undifferentiated young neurons. Subcellular Location: Nucleus. Tissue Specificity: Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas. Database links: Entrez Gene: 395802 Chicken Entrez Gene: 5629 Human Entrez Gene: 19130 Mouse Entrez Gene: 399348 Xenopus laevis Entrez Gene: 30679 Zebrafish Omim: 601546 Human SwissProt: Q91018 Chicken SwissProt: Q92786 Human SwissProt: P48437 Mouse Unigene: 741808 Human Unigene: 744931 Human Unigene: 132579 Mouse Unigene: 392678 Mouse Unigene: 26557 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. prox-1主要表達于晶狀體、心臟、肝臟、胰腺和神經(jīng)系統(tǒng)等的非內(nèi)皮細胞，在成人正常組織、胚胎淋巴管內(nèi)皮細胞或腫瘤組織的淋巴內(nèi)皮細胞上也可檢測到。移除prox-1基因后，胚胎發(fā)育時原始靜脈內(nèi)皮細胞生成淋巴管過程被阻斷，表明prox-1是淋巴管生成最基本的調(diào)控因子. |
| 產(chǎn)品圖片 |
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti- antibody (bs-2774R-BF647)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
