| 產(chǎn)品編號 | bs-2222R-BF594 |
| 英文名稱1 | Rabbit Anti-cellulase/BF594 Conjugated antibody |
| 中文名稱 | BF594標記的纖維素酶抗體 |
| 別 名 | GUN25_ARATH; Endoglucanase 25; Cellulase homolog OR16pep; Endo-1,4-beta glucanase 25; Protein KORRIGAN; Protein RADIALLY SWOLLEN 2. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 藥物及化合物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Arabidopsis Thaliana, ) |
| 產(chǎn)品應(yīng)用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 69kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Arabidopsis thaliana cellulase |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Cellulase is any of several enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis, the decomposition of cellulose and of some related polysaccharides. Cellulases break down the cellulose molecule into monosaccharides ("simple sugars") such as beta-glucose, or shorter polysaccharides and oligosaccharides. Cellulose breakdown is of considerable economic importance, because it makes a major constituent of plants available for consumption and use in chemical reactions. The specific reaction involved is the hydrolysis of the 1,4-beta-D-glycosidic linkages in cellulose, hemicellulose, lichenin, and cereal beta-D-glucans. Because cellulose molecules bind strongly to each other, cellulolysis is relatively difficult compared to the breakdown of other polysaccharides such as starch. Function: Required for cellulose microfibrils formation. Involved in cell wall assembly during cell elongation and cell plate maturation in cytokinesis. Required for secondary cell wall formation in the developing xylem. May cycle through different intracellular compartments, including plasma membrane. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Note=Cell plate. Tissue Specificity: Highly expressed in roots and stems, at intermediate levels in leaves and flowers, and at lower levels in siliques. Expressed in xylem (at protein level). Post-translational modifications: Glycosylated. N-glycosylation of KOR in the endoplasmic reticulum followed by N-glycan modifications in the Golgi are essential for catalytic activity. Similarity: Belongs to the glycosyl hydrolase 9 (cellulase E) family. Database links: UniProtKB/Swiss-Prot: Q38890.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 纖維素酶(cellulase) 它是與脫落直接有關(guān)的酶，菜豆、棉花和柑橘葉片脫落時，纖維素酶活性增加。從菜豆葉柄離區(qū)中分離出pI9.5和pI4.5(分別稱為9.5或4.5纖維素酶)兩種纖維素酶，前者與細胞壁木質(zhì)化有關(guān)，受IAA調(diào)控；后者與細胞壁降解有關(guān)，受乙烯調(diào)控。測定柑橘小葉片離區(qū)的各個不同區(qū)段中的纖維素酶活性，發(fā)現(xiàn)酶活性最高的部位是在離區(qū)的近軸端（靠近莖的0.22mm處），所以纖維素酶的活性不一定與離層細胞的分開直接有關(guān)，而可能與離層分離后的保護層發(fā)育的關(guān)系更為直接。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
