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Rabbit Anti-Phospho-MKK3(Ser218) + MKK6(Ser207) /BF350 Conjugated antibody (bs-3274R-BF350)
訂購(gòu)熱線:400-901-9800
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說(shuō) 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-3274R-BF350
英文名稱1 Rabbit Anti-Phospho-MKK3(Ser218) + MKK6(Ser207) /BF350 Conjugated antibody
中文名稱 BF350標(biāo)記的磷酸化絲裂原活化蛋白激酶MKK3/6抗體
別    名 MKK3(Phospho-Ser218); MAP Kinase Kinase 3; Dual specificity mitogen activated protein kinase kinase 3; ERK kinase 3; MAP kinase kinase 3; MAP2K 3; MAPK ERK kinase 3; MAPK kinase 3; MAPKK 3; MAPKK3; MEK 3; MEK3; Mitogen activated protein kinase kinase 3; MKK 3; MKK3; mMKK 3b; mMKK3b; MPK 3; PRKMK 3; PRKMK3; Protein kinase mitogen activated kinase 3; SKK2; zMKK 3; MP2K3_HUMAN; MP2K6_HUMAN  
規(guī)格價(jià)格 100ul/2980元 購(gòu)買        大包裝/詢價(jià)
說(shuō) 明 書 100ul  
產(chǎn)品類型 磷酸化抗體 
研究領(lǐng)域 腫瘤  信號(hào)轉(zhuǎn)導(dǎo)  細(xì)胞凋亡  生長(zhǎng)因子和激素  轉(zhuǎn)錄調(diào)節(jié)因子  激酶和磷酸酶  細(xì)胞膜受體  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
產(chǎn)品應(yīng)用 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 39kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human MKK3 around the phosphorylation site of Ser218
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.

Function:
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.

Subunit:
Binds to DYRK1B/MIRK and increases its kinase activity. Part of a complex with MAP3K3, RAC1 and CCM2. Interacts with ARRB1. Interacts with Yersinia yopJ.

Tissue Specificity:
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.

Post-translational modifications:
Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity. Phosphorylated by TAOK2.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

DISEASE:
Note=Defects in MAP2K3 may be involved in colon cancer.

Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.

Database links:

Entrez Gene: 5606 Human

Entrez Gene: 26397 Mouse

Entrez Gene: 303200 Rat

Omim: 602315 Human

SwissProt: P46734 Human

SwissProt: O09110 Mouse

Unigene: 514012 Human

Unigene: 18494 Mouse

Unigene: 100064 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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