| 產(chǎn)品編號 | bs-0455R-Bio |
| 英文名稱1 | Rabbit Anti-HSL/LIPE/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的荷爾蒙敏感脂肪酶抗體 |
| 別 名 | Hormone sensitive lipase; hormone-sensitive lipase; Hormone sensitive lipase testicular isoform; HSL; LHS; Lipase hormone sensitive; LIPE; Hormone-sensitive lipase; LIPS_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 心血管 免疫學 生長因子和激素 糖尿病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat, (predicted: Fruit Fly, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 116kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat HSL N-terminus |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: HSL ( hormone- sensitive lipase ) is an enzyme involved in hydrolysis of stored triglycerides and fatty acids; important for regulation of energy homeostasis. It has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Function: In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Subunit: Interacts with PTRF in the adipocyte cytoplasm. Subcellular Location: Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Similarity: Belongs to the 'GDXG' lipolytic enzyme family. Database links: Entrez Gene: 3991 Human Entrez Gene: 16890 Mouse Omim: 151750 Human SwissProt: Q05469 Human SwissProt: Q6NSL7 Human SwissProt: P54310 Mouse Unigene: 656980 Human Unigene: 158548 Mouse Unigene: 333679 Mouse Unigene: 10566 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. HSL又稱：敏感性甘油三酯脂肪酶，在脂肪動員中，脂肪細胞內(nèi)激素敏感性甘油三酯脂肪酶（HSL）起決定性作用，它是脂肪分解的限速酶，稱為激素敏感性脂肪酶。 腎上腺素、去甲腎上腺素、胰高血糖素等激活HSL促進脂肪動員；胰島素，前列腺素E及煙酸等抑制HSL，抑制脂肪的動員。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
