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Rabbit Anti-GRP94/RBITC Conjugated antibody (bs-0194R-RBITC)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產(chǎn)品編號 bs-0194R-RBITC
英文名稱1 Rabbit Anti-GRP94/RBITC Conjugated antibody
中文名稱 羅丹明(RBITC)標記的葡萄糖調(diào)節(jié)蛋白94抗體
別    名 HSP gp96; heat shock protein gp96 precursor; 94 kDa glucose regulated protein; ECGP; Endoplasmin; Endothelial cell (HBMEC) glycoprotein; Glucose regulated protein 94; Glucose regulated protein 94kDa; gp96; gp96 homolog; GRP 94; Heat shock protein 90 kDa beta member 1; HSP90B1; Stress inducible tumor rejection antigen GP96; TRA1; Tumor rejection antigen 1; Tumor rejection antigen gp96; ENPL_HUMAN; Endoplasmin.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 細胞生物  免疫學  神經(jīng)生物學  信號轉(zhuǎn)導  細胞凋亡  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, )
產(chǎn)品應用 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 86kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GRP94
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Glucose regulated protein 94 (GRP 94) is a resident protein of the endoplasmic reticulum (ER) and is induced by the accumulation of unfolded proteins suggesting that it might associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP 94 and other resident ER proteins including GRP 78 and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. GRP 94 is also a low affinity, high capacity calcium binding protein, though it's role, if any, in calcium regulation is not understood.

Function:
Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.

Subunit:
Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (By similarity). Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP (By similarity). Interacts with TLR4 and TLR9, but not with TLR3.

Subcellular Location:
Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Similarity:
Belongs to the heat shock protein 90 family.

Database links:

Entrez Gene: 7184 Human

Entrez Gene: 22027 Mouse

Omim: 191175 Human

SwissProt: P14625 Human

SwissProt: P08113 Mouse

Unigene: 192374 Human

Unigene: 87773 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

GRP94(G蛋白偶聯(lián)受體94)蛋白又稱GP96屬于熱休克蛋白90家組成員。由二硫鍵連接的同源二具體表達在內(nèi)質(zhì)網(wǎng)腔。內(nèi)質(zhì)網(wǎng)分子伴侶Grp94在正常細胞的內(nèi)質(zhì)網(wǎng)腔中含量較高,Grp94在內(nèi)質(zhì)網(wǎng)生成、并存留在內(nèi)質(zhì)網(wǎng)中,其表達與多種因素有關,各種應激條件下可見其表達明顯增高。細胞周期也是常見的影響細胞增殖的因素之一,因此細胞增殖過程中Grp94的表達情況具有重要意義。Grp94與細胞周期有關。
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